Karthikeyan Perumal

Medical Microbiology Lab Technician

PURE HEALTH LABORATORY- Laboratory Technologist,

ABU DHABI, Aug 2021 Jan 2023

• Molecular Biology Laboratory mainly focuses on RT-PCR Diagnosis test for

COVID-19.

• Sample pooling
• Extraction of RNA USING Generotex 94 Rotary nucleic acid Extractor
• Extraction of RNA USING ZYBIO EXM6000
• Preparation of Master Mix
• Amplification of Batching
• Amplification using BioRad cfx96
• Amplification using BioRad cfx384
• Analysis of final result

Geoscience Testing Laboratory, Dubai, UAE. Jan 2019 to July2021

Senior Microbiologist and Molecular Biologist

• Performing RT-PCR for Halal testing and GMO in food samples and cosmetics.
• q-PCR Analysis kit method
• Pork and meat species identification (HALAL

TESTING).

• GMO – Genetically Modified Organisms (Food products and Cosmetics).
• Microbiological analysis of Food samples parameters- Total Plate count,

Coliforms, Yeast & Mould, Staphylococcus aureus, salmonella, Vibrio cholera, Vibrio parahaemolyticus, Clostridium perfringens, Bacillus cereus,

Listeria monocytogens, Pseudomonos, Lactobacillus.

• Food testing Reveal kit method- E.coli o157 and listeria sp.
• Water testing including potable, swimming pool, chilled water, irrigation water, Cooling tower, Waste water and Bore well water.
• Water parameters like Total Coliforms, E.coli, Faecal coliforms,

Pseudomonas spp. S.aureus, Clostriduium perfrngens, Salmonella,

F.streptococci.

• Legionella testing using ISO Standard 11731.
• Air Monitoring- Aerobic plate count and Yeast and Mould.
• SWAB- Personnel and Environmental testing parameters like TBC,

Coliforms, E.coli, Staphylococcus aureus and Enterobactriaceae.

• Cosmetics tastings- TBC, Yeast & Mould, Candida albicans,

Staphylococcus aureus, E.coli, Pseudomonas aeruginosa.

• Food products (Dairy food, Non dairy food, Sea food, Non sea food and

Raw food materials) especially Milk, Cream, Cereals, Meat, Fish, Egg, Nuts,

Honey, Mustard, Fruits and Vegetables, Beverages, Juice, Sauce, Sesame seeds, Ready to eat.

• Water testing, Waste usual water bacterial contamination. Performing swab tests in personal, machine, equipment and other contact surfaces and MPN

Methods.

• Manage the overall functioning of the laboratory Attend to internal and external audits. Supervise the sample analysis on Microbiological parameters.
• Monitoring indoor Air quality by using passive and active sampling methods.
• Accelerated Real time and Shelf Life study and analysis of ambient food and frozen food products.
• Preparing reports and presenting results. Responsible for investigating the unusual / unacceptable results.
• Collation and analysis of results highlighting any areas of non-compliance with product specifications.
• Maintaining and review documentation such as SOP’s and test formats etc.

Maintaining good laboratory practices, Lab equipment performance and calibration.

• Participating and successfully completed proficiency Test- LGC and FEPAS other Inter Laboratory tests and External Laboratory test.