Mahmoud Ismail

Mahmoud Ismail (PhD ,MSc, BSc)

Research Assistant at Shriners Hospitals for Children.

Extensive experience in molecular biology ,biotechnology and diagnosis .

Research assistant (November 2020-present)

Shiners’ hospital for children –Research center -Montreal (QC)

Responsibilities include but not limited:

 Designing, preparing, and performing tissue, cell, and molecular biology-based approaches such as cell culture, RNA/DNA extraction, PCR, Quantitative real time(qRT-PCR),

immunoblotting, plastic/paraffin sectioning and staining , immunohistochemistry, µCT scanning, three point bending test (3-PBT), vertebra compression test, and data analysis.

 Rodent surgery: ovariectomy, Rodded Immobilized Fracture surgery.

 Helping in Writing grants and manuscripts

 Managing and organizing the stocking and supplies in the lab.

Necropsy tech- Division of Pathology-Necropsy

Charles River Laboratories, (CAN)

Responsibilities include but not limited:

 Small and large animal, anatomy, handling, restraint.

 Independently perform specialized necropsy procedures that may include but not limited to collection of tissues for special procedures, perfusion fixation, specialized dissection, blood and cerebral spinal fluid collections.

 Serve as lead/primary technician and independently perform necropsy functions and generate data on any type of study.

Research Assistant (July 2018 – March 2019)

University of Montréal, School of Social Work. (CAN)

Responsibilities

 Recruitment of participants for a research project on the settlement experiences of Syrian refugees in Montreal.

 Interviews with participants and interpretation from Arabic to English.

Researcher II (January 2016 – June 2018)

University of Alabama at Birmingham, Division of Cardiovascular Disease (USA)

Responsibilities include but not limited:

 Managing supplies and equipment in the laboratory, keeping detailed records, writing reports,

preparing, and delivering oral presentations.

 Mice handling and drugs injection,

 Cell line culture, PCR and qRT-PCR ,

 Protein, DNA and RNA extraction and isolation

 Cryosectionning,Immunohistochemistry,

 SDS-gel electrophoresis and protein western blot,

 DNA cloning, DNA-electroelution from agarose gel.

PhD, Cardiovascular Sciences (2014 – 2018)

University of Verona (Italy), Graduate School for Health and Life Sciences, Department of

Cardiac Surgery

Major: Cardioprotection against Ischemia Reperfusion Injury.

Thesis: HDAC inhibition protects cardiomyocytes by modulating expression of opioid

Receptor and inducing prosurvival autophagy by inhibiting class I HD