Bryan Castaño

PhD in Molecular Medicine (magna cum laude) with more than 7 years of research experience in molecular oncology, genomic stability, DNA Damage Response, and replication stress. Experienced in experimental design, quantitative molecular and cellular assays, genomics, protein analysis, and complex biological data interpretation. Proven track record in international research environments across Germany, Italy, and Argentina, including first-author research published in Nucleic Acids Research.

Currently expanding computational capabilities in Python for bioinformatics and data science, with particular interest in translational biomarker discovery and validation.

Career Transition & Training (2025-08 – Present)

Data science training; German language courses; completion of German naturalization; family responsibilities.

Postdoctoral Researcher — Molecular Medicine at Ulm University Hospital (2025-01 – 2025-07)

• Conducted research on DNA Damage Response and p53-dependent DNA damage tolerance mechanisms, with a focus on replication stress and genomic stability.
• Applied quantitative cellular and molecular approaches including DNA fiber spreading, BioID, and in situ proximity ligation assay (PLA) to investigate molecular mechanisms and protein interactions.
• Designed experimental strategies, interpreted quantitative biological data, supervised PhD and Master's students, and contributed to international collaborations, manuscripts, and grant applications.

PhD Researcher — Molecular Medicine at Ulm University Hospital (2019-07 – 2024-12)

• Identified a p53-dependent hierarchy in DNA damage tolerance pathway usage, resulting in a first-author publication in Nucleic Acids Research.
• Investigated DNA Damage Response, replication stress, and DNA repair using DNA fiber spreading, in situ PLA, flow cytometry, immunofluorescence, immunoprecipitation, BioID, ELISA, and Western blotting.
• Collaborated in interdisciplinary international teams and contributed to peer-reviewed publications.

Research Fellow — Marie Skłodowska-Curie Action (H2020 RISE) at University of Tor Vergata (2018-09 – 2019-05)

• Performed CRISPR-Cas9 genome editing, single-clone selection, cell culture, PCR, and molecular characterization of engineered cell models.
• Worked with immunofluorescence, electrophoresis, SDS-PAGE, and protein analysis within an international multidisciplinary research project.

Research Associate — Molecular Oncology at University of Buenos Aires (2016-06 – 2018-08)

• Investigated molecular interactions involving the tumor suppressor p14ARF, RAS oncogenic signaling, and the tumor-associated protein MAGE-A2.
• Performed gene-expression and protein-analysis experiments using molecular biology and quantitative biochemical approaches.

Scientific Communication & Educational Leadership at University of Buenos Aires and Educational Institutions (2010 – 2019)

More than eight years of university and secondary-level teaching, mentoring, academic leadership, and scientific outreach, including Molecular and Cellular Biology instruction at the University of Buenos Aires.

Commercial Operations & Sales at HSBC / Lexmark / Sprint (2006 – 2010)

Previous client-facing sales, account management, and supervisory experience, including customer relationship management, team support, training, and performance monitoring.

PhD in Molecular Medicine – Universität Ulm (2019 – 2024)

Biological Sciences Degree in Biological Sciences – Universidad de Buenos Aires (2009 – 2018)

Teaching Degree for Higher Education in Biology – Universidad de Buenos Aires (2009 – 2017)