Isha Unone

Master's student in Integrative Neuroscience with strong hands-on experience in molecular biology, immunoassays (ELISA, immunohistochemistry), and biological sample processing. Skilled in RT-qPCR, PBMC isolation, and quantitative data analysis. Experienced in fluorescence-based detection systems and assay optimization with a focus on reproducibility, sensitivity, and data reliability.

Highly motivated to contribute to diagnostic assay development and translational biomedical R&D in an industrial setting.

Student Research Assistant - German research institute (DZNE) - Magdeburg, Germany

(2024-07 - 2025-04)

• Performed high-throughput image-based analysis using ImageJ/Fiji and CellProfiler
• Processed and analyzed 100–150 biological samples/week, ensuring data consistency
• Improved workflow efficiency through structured data organization and analysis pipelines
• Supported quantitative evaluation of biological signals across large datasets

Research Assistant – Molecular & Imaging Analysis - German research institute (DZNE) - Magdeburg, Germany

(2025-03 - 2025-04)

• Processed biological samples and performed fluorescence-based quantitative analysis
• Evaluated signal responses using standardized protocols for data reliability and reproducibility
• Collaborated in optimizing experimental workflows and data acquisition processes

Research Assistant – Immunohistochemistry & Tissue Analysis - German research institute (DZNE) - Magdeburg, Germany

(2024-04 - 2024-05)

• Performed immunohistochemical staining for protein detection in biological tissue samples
• Conducted microscopy-based acquisition and quantitative image analysis
• Followed standardized protocols to ensure experimental reproducibility and accuracy

Gene Expression Analysis (PBMCs) - Research Project

(2020-03 - 2020-08)

• Isolated PBMCs and performed DNA/RNA extraction, cDNA synthesis, and RT-qPCR
• Conducted gene expression analysis with focus on data quality and reproducibility
• Documented experimental workflows and analyzed quantitative results

M.Sc. - Integrative Neuroscience - Otto-von-Guericke University, Magdeburg, Germany (2023-10)

Bachelor of Science - Molecular biology, DNA/RNA extraction, Gene expression, Biological sample processing - Sant Gadge Baba Amravati University, India (2019-06 - 2022-06)