Akhilesh Nune

Analytical Scientist with around 7 years of experience in pharmaceutical analytical and purification laboratories, specializing in impurity isolation, peptide purification, and method development to support drug discovery and development programs. Proven expertise in Preparative HPLC, HPLC/UPLC analysis, DAC column purification, and impurity profiling, including nitrosamine and peroxide-related impurities, in compliance with regulatory and quality standards. Hands-on experience with chromatography data systems such as Empower and OpenLab CDS, along with strong capabilities in instrument calibration, troubleshooting, and stability studies.

Adept at maintaining GLP-compliant laboratory operations, accurate analytical documentation, and electronic laboratory notebooks (ELN). Known for delivering high-quality, reproducible analytical results while collaborating effectively across cross-functional teams in fast-paced R&D environments.

Analytical Scientist at Dr. Reddy's laboratories (2025-03 – Present)

• Applied laboratory technical expertise in compliance with established Standard Operating Procedures (SOPs) to ensure consistency and precision.
• Conducted impurity separation using Preparative HPLC, including Nitrosamine and Peroxide-related impurities, to support quality and regulatory compliance.
• Handled chromatographic instruments and associated software such as Gilson (Trilution), Agilent (OpenLab CDS), and Hanbon (EasyChrom) for impurity isolation activities.
• Achieved successful recovery of impurities at concentrations below 2% in line with defined quality benchmarks.
• Executed purification of linear and modified peptide molecules using preparative reverse-phase HPLC.
• Method development and optimization for peptide purification.
• Utilized DAC column technology for effective purification and separation of peptide compounds.
• Executed routine instrument verification and calibration for HPLC systems, preparative HPLC units, analytical balances, and pH meters.
• Evaluated sample purity through HPLC analysis to confirm chemical integrity and quality standards.
• Oversaw downstream processing steps, including solvent removal via rotary evaporation and freeze-drying (lyophilization) to obtain purified materials.
• Size Exclusion Chromatography (SEC) fractionation by AKTA pure (Unicorn).
• Ensured measurement accuracy through systematic calibration of pH meters and Balance equipment.
• Maintained detailed records of instrument usage, sample handling, and experimental data to support traceability and compliance.
• Documented analytical results and observations in Electronic Laboratory Notebooks (ELN) for effective data management.
• Followed Good Laboratory Practice (GLP) guidelines and safety protocols to promote a compliant and secure laboratory environment.

Senior Research Chemist at SAI Life Sciences (2021-12 – 2025-03)

• Applied technical expertise in adherence to established Standard Operating Procedures (SOPs).
• Purified small-scale, intermediate, final, and library compounds to meet project requirements.
• Purified gram-level samples using DAC column techniques for effective separation.
• Processed and purified library samples with a focus on consistency and quality.
• Conducted stability studies to assess compound integrity over time.
• Diagnosed and resolved issues related to analytical instruments to ensure uninterrupted operations.
• Supported method development activities for analytical and purification processes.
• Completed post-purification activities, including lyophilization of purified compounds.
• Maintained accurate and comprehensive documentation of routine analytical work.
• Upheld laboratory operations in compliance with Good Laboratory Practices (GLP) and safety standards.

Research Associate at Aragen Life Sciences Private limited (2019-07 – 2021-11)

• Demonstrated strong proficiency in the purification and separation of intermediate and final compounds using Preparative HPLC techniques.
• Purified peptide-based molecules to meet analytical and quality requirements.
• Contributed to analytical and purification method development activities.
• Developed and optimized analytical methods for new chemical entities (NCEs) and drug discovery compounds using HPLC and UPLC systems.
• Prepared detailed analytical test reports for intermediate and final drug substances.
• Maintained accurate and well-organized documentation for routine analytical and purification activities.
• Updated and managed instrument logbooks and electronic laboratory notebooks (ELNs) in online systems.
• Collaborated with supervisors to ensure laboratory operations complied with Good Laboratory Practices (GLP) and established safety guidelines.

MS/M. Sc in Science – A. V. College of Arts, Science & Commerce (2019)

B. Sc – Girraj Government College (2017)