James Fleming
Cell biologist/research scientist
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About
Bioproduction scientist with 4 years industry experience. Skills include molecular cloning, PCR, ddPCR, mammalian cell culture, AAV gene editing, process improvement, single cell cloning, transfections, transposon technology
Experience
Scientist II – Revvity
(Horizon Discovery),
Cambridge, Bioproduction
Oct 2022 - Present
Collaborated with the R&D team to develop the next generation of transposon-based expression vectors. Planning and executing research to identify best vector design for different molecule expression. Developed and executed cloning techniques for process improvement. Optimised transposon technology for optimal integration. Lab captain since January 2021, integrating 5s standards within labs site wide with monthly auditing to manage consumable usage and to ensure standards are maintained.
Scientist I – Revvity
(Horizon Discovery),
Cambridge, Bioproduction
Sep 2019 – Oct 2022
Supported the development of the ADCC+ CHO cell line using rAAV gene editing techniques and evaluated cell line performance (growth/productivity). Required HEK-293 transfection, AAV quantification and purification. Demanded meticulous record keeping for full traceability of reagents and consumables.
Education
MSc Molecular medicine, University of East Anglia, 2018-2019: Distinction
Key Modules: Frontiers in Molecular Medicine I/II. Medicinal Biotechnology. Genetics, genomics and bioinformatics.
