Venkata Datta Paritala

Cancer Geneticist with 5+ years of combined academic and clinical experience in Next-Generation Sequencing (NGS), molecular diagnostics, and cancer genomics. Skilled in DNA/RNA extraction, qPCR, WGA, and library preparation for Illumina and Ion GeneStudio platforms, with proven ability to deliver high-quality genomic data under ISO 15189 and ISO 9001 standards. Demonstrated expertise in oncology (EGFR, BRCA1/2) and reproductive genetics (PGT-A, PGT-M) workflows, including variant analysis and data validation.

Adept at assay development, SOP optimization, and method validation to support diagnostic accuracy and regulatory compliance. Currently seeking to contribute as a scientist in a leading clinical laboratory, biotech start-up, or clinical genomics organisation, advancing precision medicine and translational research initiatives.

Research Assistant (Team Lead) - Rainbow Children's Hospital - Hyderabad

(2021-02 - 2023-06)

Specialised in Next-Generation Sequencing (NGS) using Ion GeneStudio S5 and Illumina NextSeq 500 platforms for advanced genetic testing and diagnostics.

• Isolated cfDNA and ctDNA from embryonic and maternal samples for non-invasive prenatal testing (NIPT), PGT-A, PGT-M, and cancer genomics applications.
• Prepared libraries for Whole Genome Amplification (WGA) and optimised workflows for PGT-A and PGT-M, improving assay reliability in reproductive genetics.
• Performed targeted exome sequencing for cancer panels (e.g., EGFR, BRCA1, and BRCA2), contributing to precision medicine and therapeutic interpretation.
• Executed PCR, RT-qPCR, and ddPCR assays, ensuring accurate quantitative and qualitative analyses of genomic material.
• Analysed NGS data using RStudio and GraphPad Prism, delivering actionable variant insights for clinical decision-making.
• Led method validation and authored SOPs aligned with ISO 15189 and QMS requirements, strengthening compliance and process consistency.
• Implemented TRF and ICF documentation for data integrity and GDPR compliance.
• Validated NIPT, PGT-A, PGT-M, and exome workflows across 200 samples with 100% concordance, ensuring clinical reliability.
• Managed different clinical samples using LIMS for NGS and molecular testing.
• Designed and validated a novel single-operator end-to-end NGS workflow from nucleic acid extraction to variant reporting, reducing turnaround time by 25% and error rates by 80%; subsequently authored the SOP and led its formal implementation as a standardised laboratory protocol.
• Supported laboratory automation and troubleshooting, improving throughput, reproducibility, and operational efficiency.

Consultant Scientist - Pathcare Laboratories - Hyderabad

(2020-06 - 2021-02)

• Performed FISH analysis for cancer-related genes, including TP53, EGFR, and the fusion gene BCR-ABL, achieving 100% Hybridisation signal clarity and accurate detection of chromosomal abnormalities across all analysed samples.
• Executed routine FISH testing on 200–230 patient samples per month for the detection of chromosomal aneuploidies (Trisomies 13, 18, 21) and sex chromosome abnormalities (X, Y), ensuring precise probe Hybridisation, signal enumeration, and result validation in accordance with ISO 15189 quality standards.
• Performed molecular diagnostic assays for haematologic malignancies, including JAK2 V617F mutation testing using DNA extraction (Qiagen spin-column and vacuum-based methods), followed by allele-specific PCR for the diagnosis of myeloproliferative neoplasms (MPNs).
• BCR-ABL fusion gene detection using RNA extraction, cDNA synthesis, and quantitative real-time PCR (qPCR) to support the diagnosis and therapeutic monitoring of chronic myeloid leukaemia (CML).
• Processed and analysed over 10,000 SARS-CoV-2 samples, performing RNA extraction (manual and automated Qiagen platforms) and real-time PCR, achieving 100% analytical accuracy and a fast turnaround in a high-throughput clinical environment.
• Maintained and calibrated cytogenetic and molecular laboratory instruments, managed reagent traceability and inventory, and ensured strict adherence to QMS, BSL-2 biosafety, and SOP validation for continuous compliance and audit readiness.

Clinical Research Associate - MicroLabs Pvt Ltd - Bangalore

(2019-04 - 2020-06)

• Drafted and implemented clinical trial protocols in compliance with ICH-GCP guidelines across the clinical research organisation.
• Managed site initiation, monitoring, and closeout visits, ensuring high-quality data collection and regulatory adherence.
• Trained site staff on study procedures and data integrity, enhancing operational efficiency and audit readiness.
• Oversaw ethical submissions, subject welfare, and investigational product logistics to support successful trial delivery.
• Served as the primary communication bridge between clinical site teams and the central R&D organisation, producing and presenting progress reports, protocol deviations, and data integrity summaries to senior stakeholders across departments.

Junior Lab Technician - Cytomol Advanced Diagnostics - Hyderabad

(2018-12 - 2019-02)

• Gained experience operating qPCR machines, Qubit, NanoDrop, and NGS instruments, paralleling Illumina's equipment expectations.
• Participated in NGS-based library preparation, gel electrophoresis, and basic data QC, ensuring familiarity with standard genomics workflows.
• Applied foundational molecular biology methods such as DNA/RNA quantification, amplification, and clinical diagnostics.
• Performed chromosomal karyotyping and FISH analysis using Olympus light and fluorescence microscopy for the detection of structural and numerical chromosomal abnormalities.
• Performed biochemical assays using the automated VIDAS system and ELISA-based detection for biomolecules, including Vitamin D, testosterone, and oestrogen, ensuring precise quantitative analysis and quality-controlled reporting.

BCIL trainee - Bioserve Biotechnologies - Hyderabad

(2017-12 - 2018-05)

• Analysed animal and human biological samples for genetic and microbial studies, ensuring precise data interpretation and experimental reproducibility.
• Extracted nucleic acids using multiple protocols (manual, automated, and vacuum-based) to optimize yield and purity for downstream molecular applications.
• Prepared high-quality exome libraries (SureSelect) following genomic DNA fragmentation via enzymatic digestion and ultrasonication, and validated library integrity using the Agilent Bioanalyzer for Illumina sequencing.
• Performed PCR, RT-PCR, gel electrophoresis, bacterial genotyping, and oligonucleotide synthesis for primer design, ensuring sequence accuracy and optimal amplification efficiency.
• Contributed to ISO 9001-compliant production and quality control of nucleic acid extraction buffers and reagents, maintaining strict adherence to SOPs and QA documentation in a regulated molecular diagnostics environment.

Master of Research - Cancer Biology - Nottingham Trent University (2023-09 - 2024-09)

Master of Science - Human Genetics - Andhra University (2015-04 - 2017-04)

Bachelor of Science - Biotechnology - Krishna University (2011-04 - 2015-05)