Dhanashree Shinde

Motivated researcher with strong training in Agricultural Biotechnology, Bioinformatics, Genome Editing, and NGS data analysis, seeking a research-oriented position where advanced wet-lab and computational skills can be applied to solve problems in plant, animal, and biomedical sciences.

Highly Skilled (Research – Bioinformatics & Plant Genomics) - ICAR – Directorate of Onion and Garlic Research (DOGR) - Rajgurunagar, Pune, MH

(2025-10)

Conducted genome-wide analysis of WRKY transcription factors in onion under chromium and salinity stress using integrated wet-lab (RT-qPCR) and bioinformatics approaches.

• Conducted genome-wide analysis of WRKY transcription factors in onion under chromium and salinity stress using integrated wet-lab (RT-qPCR) and bioinformatics approaches (TBtools, MEGA, MEME, STRING)
• Performed genome-wide identification and characterisation of the WRKY gene family in Allium cepa under abiotic stress conditions
• Carried out in-silico annotation, including chromosomal localisation, gene duplication, synteny, collinearity, and Ka/Ks analysis
• Constructed phylogenetic trees and analysed conserved motifs, gene structure, and physicochemical properties
• Identified cis-regulatory elements (PlantCARE), predicted subcellular localisation, and performed GO enrichment analysis
• Developed protein–protein interaction (PPI) networks using STRING and Cytoscape
• Performed RNA isolation, cDNA synthesis, and RT-qPCR-based expression profiling
• Applied statistical analysis and visualised results using TBtools
• Integrated wet-lab and computational approaches to identify candidate genes for abiotic stress tolerance
• Performed 16S rRNA gene-based microbial identification and diversity analysis
• Isolated microbial DNA and conducted PCR amplification of the 16S rRNA gene using universal primers
• Analysed sequencing data for taxonomic classification and phylogenetic relationships
• Constructed phylogenetic trees using MEGA to study evolutionary relationships among microbial species
• Conducted sequence alignment and similarity analysis (BLAST, NCBI databases)
• Assisted in studying microbial communities associated with plant stress conditions

Research Scholar (MSc) - ICAR – Directorate of Onion and Garlic Research (DOGR) - Pune, MH

(2024-02 - 2024-02)

• Performed genome editing experiments, including molecular cloning, plant transformation, and regeneration of transgenic plants
• Designed and optimised CRISPR–Cas guide RNAs for targeted genome editing
• Designed gene-specific primers and optimised PCR and RT-PCR conditions
• Analysed Sanger sequencing data for mutation detection and sequence validation
• Processed and interpreted Next-Generation Sequencing (NGS) datasets
• Conducted phylogenetic analysis and gene structure prediction using bioinformatics tools
• Retrieved, curated, and analysed biological data from public databases (NCBI, UniProt, etc.)

Internship Researcher - CSIR – National Chemical Laboratory (NCL) - Pune, MH

(2023-06 - 2023-06)

• Worked on computer-aided drug discovery (CADD) projects
• Performed virtual screening and molecular docking to evaluate ligand–protein interactions
• Analysed computational simulation data for lead compound optimisation
• Collaborated with interdisciplinary teams, integrating computational and biochemical insights

Training Researcher (B.Tech) - ICAR – National Institute of Abiotic Stress Management (NIASM) - Baramati, MH

(2022-01 - 2022-01)

• Hands-on training in the Animal Biotechnology laboratory and animal experimentation facilities
• Exposure to animal housing systems, data recording, and ethical handling
• Worked in the hydroponics unit growing maize, oats, barley, wheat, rice, and sorghum
• Conducted fermentation and silage production for animal feed
• Estimated biochemical parameters, including BUN, AST, ALT, calcium, and phosphorus
• Participated in conferences on Animal Nutrition, Physiology & Breeding

M.Sc. - Bioinformatics - Walchand College of Arts & Science, Solapur (2024)

B.Tech - Agricultural Biotechnology - Aditya College of Agricultural Biotechnology, Beed (2022)

HSC - Mahatma Jyotiba Phule College, Kurduwadi, Solapur (2018)

SSC - Nutan Vidyalaya, High School, Kurduwadi, Solapur (2016)