Jay Khaptawala
BIOPROCESS & FERMENTATION SCIENTIST
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About
Biopharma and fermentation scientist with 4+ years of end-to-end bioprocess experience — microbial strain handling, upstream fermentation, downstream processing, and GMP-compliant manufacturing at 5 L through 20 KL, across E. coli, Salmonella typhi, and Bacillus expression systems.
Experience
Senior Research Associate — Upstream Fermentation - Zero Cow Factory - Surat, India
(2025-07)
Managed full upstream workflow from strain isolation through 5.0 L bioreactor runs for recombinant protein production using E. coli intracellular expression systems. Designed and executed fed-batch fermentation strategies achieving higher biomass density; troubleshot deviations using root-cause analysis to maintain batch consistency.
- Strain isolation & screening
- Culture maintenance & preservation
- Glycerol stock management
- Inoculum & seed culture development
- Shake flask optimization
- Media & induction strategy development
- Clone screening & selection
- Fed-batch design & execution
- Batch record preparation
- DSP coordination
- Eppendorf BioFlow 120 (5 L)
- Fermex in-situ 50 L
- CIP / SIP operations
- DO / aeration / agitation Control
- Deviation troubleshooting
- SOP compliance
Senior Executive — Production Officer - Arkray Healthcare Pvt. Ltd. - Sachin, Surat
(2023-07 - 2025-06)
Produced Salmonella typhi antigen dye for Widal diagnostic kit manufacturing — operating 5 × 18 L fermenters under cGMP guidelines. Maintained culture integrity via master/sub-plate management; full BPCR documentation every batch ensuring GDP compliance and traceability.
- 5 × 18 L fermenter operation
- Master plate & sub-plate management
- Growth kinetics monitoring
- Aseptic culture transfers
- Biosafety Cabinet (BSC) operations
- Centrifuge — biomass separation
- Antigen dye recovery
- In-process sampling & QC
- Buffer & solution preparation
- BPCR — every batch
- cGMP & GDP compliance
- SOP adherence & review
- Deviation reporting
- Batch traceability records
Production Junior Officer - Zytex Biotech Pvt. Ltd. - Savli Biotech Park, Baroda
(2022-02 - 2023-06)
- CIP — Clean-in-Place
- SIP — Steam-in-Place
- Empty sterilization
- Pressure test — leak detection
- FIT (During a strain changeover)
- 100 L to 20 KL prep
- Bacillus sp. high-biomass culture
- 100 L seed fermentation
- 10 KL & 20 KL production scale
- Process parameter control
- Batch execution & BMR
- SOP & GMP compliance
- Microfiltration
- Spray drying — powder recovery
- Blending & sifting
- Packing & labelling
- Cold storage transfer
- Yield tracking
Trainee Officer — QC Microbiology Department - Macleods Pharmaceuticals Ltd. - Sarigam, Bhilad
(2021-11 - 2022-01)
- Environmental monitoring
- MLT (Microbial Limit Test)
- RM, IP, FG Material sample testing
- Sterility Test
- GDP — Documentation Practice
- GMP — Manufacturing Practice
- GLP — Laboratory Practice
- ALCOA & ALCOA+ principles
- Equipment & Facility Logs
- Up-to-Date QC Manuals
Education
M.Sc - Biotechnology - P.P. Savani University, Surat (2019 - 2021)
B.Sc - Biotechnology (Hons.) - P.P. Savani University, Surat (2016 - 2019)
