Komal Thorat

Research Scientist with 7+ years of experience in plant molecular genetics, genomics, and marker-assisted breeding in ICAR-funded national research programs. Proven expertise in QTL mapping, GWAS, SNP/SSR genotyping, candidate gene discovery, and trait dissection in grapevine. Strong industry-aligned skills in high-throughput genotyping, data analysis, SOP-based lab operations, cross-functional collaboration, and regulatory documentation.

Published researcher with hands-on experience translating genomic data into breeding-ready markers.

Senior Research Fellow (Research Scientist Role) - ICAR – National Research Centre for Grapes - Pune

(2023-01)

Led molecular genetics experiments for trait discovery and validation under ICAR/DBT/DST projects

• Designed and executed workflows for DNA/RNA extraction, PCR, qPCR, SSR and SNP genotyping
• Conducted GWAS and QTL mapping for grape bunch and berry traits using R, TASSEL, and ICIMapping
• Identified and prioritized candidate genes from significant genomic regions
• Integrated phenotypic and genotypic datasets for trait-marker association
• Performed SNP filtering, annotation, and genotype quality control
• Ensured SOP compliance, data integrity, and instrument calibration
• Prepared technical reports, grant deliverables, and research manuscripts
• Mentored junior researchers and coordinated lab-field activities

Junior Research Fellow - ICAR – National Research Centre for Grapes - Pune

(2019-08 - 2022-06)

• Executed high-throughput genotyping using SSR and SNP markers
• Constructed linkage maps and performed QTL analysis for complex agronomic traits
• Conducted qPCR-based expression profiling of candidate genes
• Collected and analyzed field phenotyping data under vineyard conditions
• Used JoinMap, MapDisto, QTL Cartographer, and R/qtl for mapping and analysis
• Supported manuscript writing and regulatory submissions
• Maintained accurate lab records and field documentation

Young Professional-II - ICAR – National Research Centre for Grapes - Pune

(2018-09 - 2019-06)

• Worked on gene cloning and functional validation of abiotic stress-responsive genes
• Performed plant tissue culture, somatic embryogenesis, and transformation support
• Assisted in quality control, lab inventory, and workflow optimization
• Built foundation in industry-relevant stress tolerance research

Ph.D. - Biotechnology - Savitribai Phule Pune University (SPPU)

M.Sc. - Agricultural Biotechnology - Dr. Panjabrao Deshmukh Krushi Vidyapeeth (PDKV)

B.Sc. - Agricultural Biotechnology - Dr. Panjabrao Deshmukh Krushi Vidyapeeth (PDKV)