M B

Experienced research associate with expertise in clone development, recombinant protein expression, and purification. Proficient in plasmid design, DNA/RNA detection, and project management. Skilled in mammalian cell culture and bioinformatics.

Committed to scientific excellence and contributing to impactful research. Strong background in experimental design, data interpretation, and laboratory best practices. Experienced in maintaining thorough documentation and ensuring compliance with quality and research standards.

Research Associate - Abhignya Biotechnology Consultants

(2025-07)

• Assisted in antimicrobial and anti – fungal research, analyzing efficacy of agents.
• Utilized advanced bioinformatics tools for data analysis.
• Executed molecular biology techniques (DNA/RNA extraction, PCR, gel electrophoresis).
• Worked on cloning and sequencing genetic materials.

Research associate - Revelations Biotech

(2024-08 - 2025-06)

• Generate plasmid preps and characterize by RE digests and sequencing
• Prokaryotic and eukaryotic cloning, protein expression and purification, enzyme assays, SDS-PAGE.
• Independently design complexplasmids and executethe cloning, using either PCR, restriction enzymes,or assembly to a high technical standard
• Performing DNA/RNA detectionusing fully automatedand semi – automated systems
• Projects handled on – (Pichiapastoris) yeast, E.coli,Basilus subtilis

Executive - R&D Procell Biologics Pvt Ltd

(2022-02 - 2024-06)

Execution of Clone Development, Recombinant Protein Expression Activities and purification by batch mode:

• Gene Amplification, Restriction Digestion, Ligations, and Transformation
• SDS-PAGE, and CloneCharacterization and mammalian cell culture characterization experiments.
• Performing western blotting technique.
• Performing DNA and RNA isolationin cell.
• Knowledge on spectromax plate read , Knowledge on flow-cytometer to analyze the characteristics of cell.
• Perform protein quantification of insulin glargine, lispro and human insulin by using of ELISA
• Analyzing the growth characteristics and culture condition of cells and isolating the contamination causes.
• Performing bradford assay for total protein estimation in required sample
• Culture and cryopreservation of mammalian cell lines
• Preparation of Standard Operating Procedures
• Isolation of DNA from Plants,Onion, Chicken Livercells, and AnimalTissue
• Techniques of mammalian cell culture
• Performing purification of TEv protease in Batch mode
• Performing purification of capase-2 in Batch mode
• Operating Cation, Anionexchange and affinitychromatography in Batchmode.
• Basic knowledge on analytical tools.

Internship - Abhigna Biotechnology consultants

(2021-08 - 2022-01)

• Assisted in antimicrobial and anti – fungal research, analyzing efficacy of agents.

Project - Azymes-Biosciences - Banglore, India

(2021-10 - 2021-11)

Biosynthesis of selenium nanoparticles from Bacillus spp and its biological activities

Internship - CSIR-Indian institute of ChemicalTechnology (CSIR-IICT) - Hyderabad, India

(2019-01 - 2019-03)

• Chemoinformatics – drug designing

Masters - Biotechnology - Oxford College of Science and Arts (2019 - 2021)

Bachelors - Biotechnology - Bhavans Vivekananda Degree College of Science Humanities (2016 - 2019)