Cytogenetic Technologist III - University of Maryland FPI Inc. - Baltimore, Maryland, United States
(2014-06)
- Lead technologist on "BEAT AML" clinical trial studies. Responsibilities include performing chromosomes and FISH testing within specific time guidelines, troubleshooting and editing protocols, accurately completing the initial and detailed forms for the patient in the BEAT study and making the composite power-point presentations for the director
- Reviewing the cases of other technologists and monitoring the abnormalities closely for the director to sign out.
- Perform Oncology Cytogenetic set-up, harvest, analysis and report writing on the bone marrow, peripheral blood and solid tissue specimens
- Identify technical problems and provide timely troubleshooting and resolution
- Participate in CAP proficiency testing (FISH, chromosomes, POCs and SNP microarray)
- Teach Master's students in Genetic Counseling every year for a period of two months
- Separation of Plasma cells with CD138 markers for diagnosis of Multiple Myeloma
- Prepares PowerPoint slides and assists the director in leukemia conferences
- Lead technologist in analyzing microarray cases and assists the director in reporting and trains others in microarray
- Assists the director in research projects for compilation of chromosomal, FISH and microarray data
Cytogenetic Technologist - LabCorp - Manhattan, New York, United States
(2012-11 - 2014-06)
- Performs routine culture, harvest
- Prepare slides of cell cultures following standard procedures
- Stain slides to make chromosomes visible for microscopy
- Microscopic analysis of chromosomes to detect structural and numerical abnormalities and summarize test results and report to appropriate authorities
- Input details of specimens into logs or computer systems
- Also prepares solutions and reagents, maintains laboratory records
- FISH analysis
Cytogenetic Technologist - Texas Tech University Health Science Center - Lubbock, Texas, United States
(2009-07 - 2012-10)
- Test development: Methodology development, test validations. Interpretation, diagnosis, troubleshooting, problem-solving and quality control
- Case review: Maintaining quality and accuracy requirements, resulting, pre-review, preparation of all patients' reports before medical geneticist sign-out
- Professional experience: Prenatal diagnosis by FISH, cell and tissue culture techniques, wet lab and dry lab techniques and chromosome identification by following ISCN 2009 Nomenclature
- Peripheral blood and bone marrow culture and harvest, staining, microscopic chromosomal analysis, karyotyping using computer system (Cytovision software)
- Diagnosis and interpretation of micro-deletions, constitutional blood, bone marrow, and hematological malignancies related to molecular and FISH testing
- Molecular genetics includes working on ALL (adult and child), CLL, CML, MDS, MM, AML panels. Individual probes include working on enumeration probes, deletion probes, break-apart probes, translocation dual-fusion probes, inversion probes and fusion-deletion probes
- Cell culture and harvest for FISH, interpretation of Fluorescent in situ hybridization (FISH) signals using multicolor fluorescence tagged with different color flourochromes for detection of disease-associated abnormalities such as translocations and sub-microscopic deletions, numerical and structural abnormalities, and sex determination in interphase cells using Olympus fluorescent and manual fluorescent photo-microscopy.
- Specimen Preparation includes pre-treatment of amniotic fluids and urine specimens (urovysion), dropping to make slides for hybridization followed by washing
- Genomic DNA isolation from whole blood (daily routine), tissues and tissue culture cells, Preparation of Agarose gels and Agarose gel electrophoresis, Restriction digestion of genomic DNA, Enzymatic Labeling of genomic DNA and interpretation of data.
- Urovysion by FISH includes detection of aneuploidy for respective chromosomes in urine specimens from persons with hematuria suspected of bladder cancer. Optimized a standard protocol for UroVysion
Research Assistant - William Paterson University - NJ, USA
(2009-01 - 2009-06)
Nitric Oxide as a signaling molecule in Anuran Metamorphosis (2009). Analyze the role of Reactive Oxygen Species (ROS) [Nitric Oxide as a signaling molecule] in metamorphosis of tadpoles (Xenopus Laevis)
- Immunohistochemistry for localization of NO (Nitric Oxide) and NO (nitric oxide) producing cells
- Silver staining and Rhodamine 123 staining for localization of mitochondrial rich cells
- Confocal microscopy for detection of localized cells
- NADPH-Diapharose histochemistry for detection of mitochondria cells which are the source of NO producing cells in the tail epidermis of whole mounts of Xenopus laevis
- Assisted PI in conducting various assays, Maintenance of protocols and logbooks, lab equipment and chemicals
- Responsible for conducting the assays such as Elisa, RT-PCR, Immunohistochemistry, Silver staining and Western Blot techniques independently
- Statistical analysis of the data obtained
Research Assistant - William Paterson University - NJ, USA
(2007-01 - 2007-12)
Antibody Response in Mice
- Evaluation of antibody response to Ectromelia in mice while administering an antiviral drug
- Elicited immune response in mice model using peptide antigen
- Analysis of serum for antibody response using ELISA
