Ramkumar Sundar

Innovative and results-driven Molecular Biologist with proven expertise in managing research projects from concept to completion. Adept in advanced molecular and biochemical techniques, including nucleic acid isolation, PCR, Sanger sequencing, ELISA, and western blotting. Demonstrated ability to analyze complex datasets, interpret experimental outcomes, and deliver high-quality scientific reports.

Passionate about driving translational research and contributing to cutting-edge discoveries in molecular biology and biotechnology.

Research & Development - Micro Crispr Pvt.Ltd

(2024-04)

• Designed plasmid constructs and siRNA sequences to support ASO/siRNA transfection studies, including CFB-targeted approaches for Dravet syndrome research.
• Performed isolation and handling of in vitro samples treated with ASO and siRNA for downstream gene expression studies.
• Performed gene knockout and knockdown validation assays using molecular and analytical techniques including qPCR, ddPCR, dPCR, Western blot, and ELISA.
• Performed sequencing, analysis, and optimization of multiple AAV serotypes for research and translational applications.
• Designed and analyzed siRNA for off-target prediction using CHOPCHOP and CRISPOR.
• Developed and optimized AAV gene therapy workflows for SMN1 programs using PCR, qPCR, ddPCR, ELISA, SDS-PAGE, and Western blot assays.
• Utilized advanced molecular biology techniques including recombinant DNA and RNA manipulation.
• Supported assay development and data analysis in a regulated R&D laboratory environment.

Molecular Biotechnologist - Lifecell International Pvt.Ltd

(2020-01 - 2023-12)

• Identified genetic mutations associated with the development of various diseases in single-nucleotide variants (SNVs) using Sanger sequencing.
• Identification of GCC Repeats in FRAGILE X at fragment analysis (Prenatal Samples and blood).
• Genetic analysis of particular genes like HBB, CFTR and GALT.
• Conducted extensive research on DNA sequencing and PCR techniques in infectious diseases like TB, TORCH, HPV, and COVID-19.
• Proficient in GeneXpert (MTB, COVID19, TORCH, HCV, HPV).

M.Tech - Biotechnology - Anna University, Chennai (2023-01 - 2025-12)

B.Tech - Biotechnology - Anna University, Chennai (2016-01 - 2020-12)