Rutuja Nandre
Doctor of Philosophy in Bio and Medical Big-Data
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About
Research experience in cloning gene fragments on pUC19, 1860, PUC series vectors, commercial plasmid backbones, CRISPR Cas9 genome editing system in mushroom NHEJ-HR, protoplast mediated transformation, GFP expression under the three native promoter Cassette, PCR, RT-PCR, RT-qPCR, Fluorescence microscopy, GFP signal detection, Sanger sequencing analysis.
Experience
Ph.D Student - Gyeongsang National University - South Korea
(2021-02 - 2025-08)
Research in gene editing and mushroom transformation
- Cloning DNA fragments on pUC19, 1860, PUC series vectors, commercial plasmid backbones
- CRISPR Cas9 genome editing system in mushroom NHEJ-HR
- Protoplast mediated transformation and regeneration
- PyrG gene deletion with insertion of 2.5 kb fragment (GFP cassette)
- Sanger sequencing confirmation and analysis of NHEJ-HR mechanism of transformants
- Fluorescence microscopy detection of GFP
- Successful genome editing at the target site by CRISPR-Cas9 RNP ribonucleoprotein mediated genome editing method
- Experiences on Agrobacterium tumefaciens mediated transformation in mushroom
Masters Project Researcher - CSIR National Chemical Lab - Pune, India
(2018-01 - 2019-01)
Master last year project in Chemical sciences division Microbio lab working on final cultures
- Synthesis of nanoparticles from fungi
- Study of Effect of Nanoparticles for Enzyme Protease production
- Synthesized nanoparticles from yeast with supernatant and during growth (fermentation)
- Checked effect of NPs on enzyme protease production
- Application in Textile and MB dye degradation in mushroom
Education
Doctor of Philosophy - Bio and Medical Big-Data - Gyeongsang National University (2021-02 - 2025-08)
Masters - Bachelor - School of Nanoscience and technology - Shivaji University Kolhapur (2014-01 - 2017-12)
Masters - School of Nanoscience and Biotechnology - Shivaji University Kolhapur (2017-01 - 2019-12)
Post graduation diploma - Bioinformatics - Shuvaji University Kolhapur (2017-01 - 2018-12)
