Rutuja Nandre

Research experience in cloning gene fragments on pUC19, 1860, PUC series vectors, commercial plasmid backbones, CRISPR Cas9 genome editing system in mushroom NHEJ-HR, protoplast mediated transformation, GFP expression under the three native promoter Cassette, PCR, RT-PCR, RT-qPCR, Fluorescence microscopy, GFP signal detection, Sanger sequencing analysis.

Ph.D Student - Gyeongsang National University - South Korea

(2021-02 - 2025-08)

Research in gene editing and mushroom transformation

• Cloning DNA fragments on pUC19, 1860, PUC series vectors, commercial plasmid backbones
• CRISPR Cas9 genome editing system in mushroom NHEJ-HR
• Protoplast mediated transformation and regeneration
• PyrG gene deletion with insertion of 2.5 kb fragment (GFP cassette)
• Sanger sequencing confirmation and analysis of NHEJ-HR mechanism of transformants
• Fluorescence microscopy detection of GFP
• Successful genome editing at the target site by CRISPR-Cas9 RNP ribonucleoprotein mediated genome editing method
• Experiences on Agrobacterium tumefaciens mediated transformation in mushroom

Masters Project Researcher - CSIR National Chemical Lab - Pune, India

(2018-01 - 2019-01)

Master last year project in Chemical sciences division Microbio lab working on final cultures

• Synthesis of nanoparticles from fungi
• Study of Effect of Nanoparticles for Enzyme Protease production
• Synthesized nanoparticles from yeast with supernatant and during growth (fermentation)
• Checked effect of NPs on enzyme protease production
• Application in Textile and MB dye degradation in mushroom

Doctor of Philosophy - Bio and Medical Big-Data - Gyeongsang National University (2021-02 - 2025-08)

Masters - Bachelor - School of Nanoscience and technology - Shivaji University Kolhapur (2014-01 - 2017-12)

Masters - School of Nanoscience and Biotechnology - Shivaji University Kolhapur (2017-01 - 2019-12)

Post graduation diploma - Bioinformatics - Shuvaji University Kolhapur (2017-01 - 2018-12)