Santhosh Kumar
Genomics, NGS, Sanger Sequencing,
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About
Molecular biologist with over 14 years of experience in genomics, DNA-based authentication, and translational research in ethnopharmacology and botanical science. Specialized in high-throughput sequencing (NGS, Sanger), microbiome analysis, with a strong track record in leading interdisciplinary projects focused on quality, identity, and safety of bioactive compounds and natural therapeutics. Experienced in developing DNA barcoding and genomic workflows for botanical ingredient and microbial authentication, including the design of marker-based tools for quality assurance.
Proven ability to manage cross-functional teams, mentor junior researchers, and collaborate with international institutions.
Experience
Genomic Scientist at Herbalife International India Pvt Ltd (2023-11 – Present)
Botanical and microbial identification testing of raw materials, finished products, and post-market products using Sanger sequencing and next-generation sequencing (NGS) techniques. Optimized methodologies for diverse sample types and prepared standard operating procedures.
- Botanical and microbial identification testing of raw materials, finished products, and post-market products using Sanger sequencing and next-generation sequencing (NGS) techniques
- Optimised methodologies for diverse sample types in both Sanger (Applied Biosystems) and NGS platforms (Illumina)
- Prepared standard operating procedures (SOPs), test methods, certificates of analysis, and comprehensive technical reports
- Performed metagenomic analysis and NGS library preparation for microbial community profiling
- Supported regulatory compliance efforts and contributed to successful internal and external audits (NABL: ISO/IEC 17025; 2017)
- Collaborated cross-functionally with QA, R&D, and analytical teams; provided training and mentorship to junior staff
- Engaged in scientific communication through workshop, seminars participation, abstract presentations, and manuscript development
Postdoctoral Research Scientist at Chulalongkorn University (2019-04 – 2023-09)
Led a project team focused on developing a DNA-based authentication system for Thai herbs used in the high-end herbal industry. Conducted field studies and performed troubleshooting for DNA analysis.
- Led a project team focused on developing a DNA-based authentication system for Thai herbs used in the high-end herbal industry
- Conducted field studies, collected herbal product samples, and performed troubleshooting for DNA isolation, PCR, and next-generation sequencing analysis
- Prepared grant proposals, project reports, scientific manuscripts, as well as abstract presentations
- Supervised undergraduate, master's level, and PhD researchers
Visiting Research Fellow at University of Guelph (2015-03 – 2015-08)
Worked as part of the TRU-ID project in assessing species admixture in botanical products. Applied PCR applications and high throughput sequencing analysis.
- Worked significantly as part of the TRU-ID project, in assessing species admixture in botanical products
- PCR application and troubleshooting, and analysis of high throughput sequencing
- Prepared herbarium specimens; applied bioinformatics tools such as NCBI Blast, Bioedit & Clustal W (Multiple Alignment techniques); Codoncode, MEGA and designing the primers and mini-barcodes
- Collaborated with international scientists and regulatory partners to promote transparency and traceability in the botanical products
- Enhanced skills in global research communication, cross-cultural scientific collaboration, and presentation of findings to diverse stakeholders
Junior and Senior Research Fellow at School of Ecology and Conservation (SEC), University of Agricultural Sciences-GKVK (2012-04 – 2018-12)
Involved in the collection of medicinal plants, identification, and preparation of Herbarium specimens. Designed and developed DNA markers for molecular authentication of medicinal plant species.
- Involved in the collection of medicinal plants, identification, and preparation of Herbarium specimens
- Designed and developed DNA markers for the molecular authentication of medicinal plant species and assessment of genetic diversity
- Applied PCR-based techniques for species validation and authentication of raw herbal materials traded in local and regional markets
- Assisted in lab management, reagent preparation, sample documentation, and equipment maintenance
Research Assistant at ICAR-Indian Institute of Horticultural Research (IIHR) (2011-08 – 2012-03)
Explored the potential of RNAi in insect pest management by working to produce a transgenic tomato with virus and insect pest-resistant diseases.
- Explored 'the potential of RNAi in insect pest management' by working to produce a transgenic tomato with virus and insect pest-resistant diseases
- Trained in molecular cloning, bacterial transformation, blotting techniques and various bioassays; also field trials
Education
PhD in Biotechnology – Kuvempu University (2013-12 – 2018-12)
MSc in Biotechnology – Kuvempu University (2009-06 – 2011-05)

