Shilpa

Dedicated biotechnology researcher with strong hands-on experience in CRISPR/Cas9 genome editing, molecular biology, tissue culture, and plant transformation. Skilled in experimental design, troubleshooting, cloning workflows, and scientific documentation. Seeking opportunities in research labs, biotech companies and academic support roles.

Doctoral Researcher - Biotechnology (CRISPR/Cas9) - REVA UNIVERSITY, BANGALORE / ICAR-IIHR, BANGALORE - Bangalore

(2023)

Deputed to ICAR-IIHR, Bangalore for research on genome editing to induce facultative parthenocarpy in tomato.

• Working on genome editing to induce facultative parthenocarpy in tomato by targeting SAG39 and NAP2 genes.
• Designed gRNA constructs using MtU6 promoter; performed cloning in pBSK and Cas9 insertion in pBI121.
• Conducted PCR, RNA isolation, cDNA synthesis, restriction digestion, and gel-based validation.
• Performed Agrobacterium-mediated transformation and tissue culture steps.
• Submitted a short communication manuscript and literature reviews covering SAG genes, senescence pathways, and CRISPR applications.
• Managed lab documentation, reagent planning, and experiment optimization.

Research Assistant - PRIMER BIOTECH - Hyderabad

(2018-02 - 2020-03)

• Microbial identification
• Total DNA/RNA extraction and purification.
• Molecular cloning

Junior Research Fellow - CCMB (Center for Cellular and Molecular biology) - Hyderabad

(2010-12 - 2011-03)

• Synthesis of Platelet lysate from cord blood which can be used as a substitute for Fetal Bovine Serum
• Basic Animal tissue culture techniques which include Cell culture of primary and established cell lines; isolation of primary cells; transfection of cells with plasmids and fluorescence microscopy
• Molecular cloning

Research Executive - BIOTECH DESK PVT LTD - Hyderabad

(2010-04 - 2010-12)

• Total DNA/RNA extraction and purification.
• Molecular cloning through DNA isolation, PCR, restriction enzyme digests, ligation, cell transformation, and PCR screening.
• Construction and isolation of plasmid vectors used for gene expression studies
• Protein expression
• Genomic DNA library construction
• Site directed mutagenesis

Trainee Research Assistant - CLONEGEN BIOTECHNOLOGY PVT.LTD - Noida

(2009-08 - 2010-03)

• Basic Microbiology techniques
• Molecular cloning
• Teaching undergraduate students

Ph.D - Biotechnology - REVA University (2023)

M. Tech - Biotechnology - JNTU (2016)

B. Tech - Industrial Biotechnology - Bharath University (2009)