PhD in Biotechnology with expertise in molecular biology, recombinant DNA techniques, and mammalian cell-based experimental systems
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PhD in Biotechnology with strong expertise in molecular biology, recombinant DNA techniques, and mammalian cell-based experimental systems. Experienced in gene amplification, PCR-based cloning workflows, transfection, stable cell line generation, and expression analysis of recombinant proteins in mammalian systems. Hands-on experience with HEK293T and other adherent cell lines, plasmid handling, protein characterization, and cell-based functional assays.
Skilled in translating molecular constructs into functional biological systems, with a strong interest in viral vector design, clone development, and biologics research for therapeutic applications.
Post-Doctoral Research Fellow (IOE-PDRF) at University of Hyderabad (2024-10 – 2025-03)
Engaged in the project deciphering the role of Mycobacterium tuberculosis (M.tb) secreted factors on the dynamics of HIV pathogenesis. Involved in understanding the role of candidate M.tb proteins in HIV-TB co-infection using various molecular and cell biological approaches. Generated various truncated mutant constructs to assess their subcellular localization in the host cells.
Purified M.tb proteins for biochemical studies in vitro. Effect of these M. Tuberculosis proteins on host cells studied using transient transfection and infection studies with HIV and M. smegmatis to establish the HIV-M.smegmatis co-infection model.
PhD Student at University of Hyderabad (2018-01 – 2024-12)
Studied the molecular interaction of Epap-1, small molecular mimics of Epap1, and novel small molecules with the Spike-protein of SARS-CoV-2 and developed an entry inhibitor against SARS-CoV-2.
Advanced three research objectives: 1) An analysis of the effect of Epap-1 on the SARS Cov-2 Spike protein and ACE2 interaction and virus entry.
2) Evaluation of efficacy of small molecule mimics of Epap-1 on SARS-Cov-2 RBD protein and ACE2 interaction and virus entry.
3) Development of novel dicumarol inhibitors of viral entry through RBD interaction. Epap-1 protein showed inhibition against RBD and Spike protein of SARS-CoV-2. Epap-1 interferes with Spike and RBD interaction with ACE2, suggesting a possible mechanism of the antiviral environment during pregnancy.
Epap-1 small molecular mimics having pyridine and dicoumarol as pharmacophores exhibited significant inhibition of the interaction between the ACE2 receptor with RBD and S-protein.
Assistant Professor, Department of Genetics at Aurora Degree College (2018-01 – 2018-08)
Project JRF at Birla Institute of Technology (BITS Pilani), Hyderabad Campus (2017-08 – 2018-01)
Project Title: Mechanistic basis of abnormal neurogenesis due to HSAN1E- associated DNA methyl transferase 1 (Dnmt1) mutation and pharmacological intervention of phenotypic correction.
Master's Dissertation at Institute of Genetics and Hospital for Genetic Diseases, Osmania University (2014-01 – 2015-12)
Title: Cytogenetic Studies in Primary Amenorrhea
Post-Doctoral Research Fellowship (IOE-PDRF) in Biotechnology and Bioinformatics – University of Hyderabad (2024-10 – 2025-03)
PhD in Biotechnology & Bioinformatics – University of Hyderabad (2018-01 – 2024-12)
M.Sc. in Genetics – Osmania University (2013-01 – 2015-12)
B.Sc. in Life Sciences – Bhavan's Vivekananda College of Science Humanities &Commerce, Osmania University (2010-01 – 2013-12)
