Senior Technical Assistant (R&D) at Mahesh Value Products Pvt. Ltd. (2023-06 – Present)
Hands-on experience in HPLC mobile phase preparation, bioreactor operation, sample analysis, and microbiological testing with compliance to food safety standards.
- Hands-on experience in HPLC mobile phase preparation and double injection techniques to ensure high-precision analytical results.
- Operate and maintain a 750 L airlift bioreactor, optimizing process parameters for consistent bioprocess performance.
- Monitor and regulate boiler and chiller systems to maintain controlled environmental conditions for experiments.
- Culture and maintain plant hairy root cultures across various media using bioreactors ranging from 250 mL to 15 L.
- Prepare and process samples for GC-MS and HPLC analysis with a focus on accuracy, purity, and reproducibility.
- Design and develop customized bioreactors (250 mL–20 L) ensuring optimal aeration, mixing efficiency, and sterility.
- Conduct microbiological analysis of raw materials (RM), work-in-progress (WIP), bulk before transfer (BBT), and finished products.
- Ensure compliance with food safety standards such as FSSAI and ISO 22000.
- Perform shelf-life studies and routine quality control testing.
- Monitor and control microbial contamination throughout production processes.
- Maintain laboratory operations in accordance with Good Laboratory Practices (GLP).
- Support product development activities and pilot-scale trials.
- Assist in yeast culture propagation and monitor growth parameters.
- Conduct hazard analysis and monitor Critical Control Points (CCPs) and Operational Prerequisite Programs (OPRPs) as per HACCP guidelines.
- Maintain accurate documentation and generate reports using ERP systems such as Navision.
- Ensure hygienic conditions and maintain a safe laboratory environment.
- Implement strict laboratory safety protocols, maintain SOP documentation, and ensure proper calibration of analytical instruments.
- Handle procurement activities and vendor negotiations for laboratory chemicals, glassware, and equipment.
- Conduct monthly inventory audits to ensure efficient supply chain management within the research facility.
Project Assistant (Part-time) at A.V.V.M. Sri Pushpam College (Autonomous) (2021-01 – 2023-05)
Conducted molecular and biochemical studies including PCR, agarose gel electrophoresis, and tissue culture work with focus on data integrity and accuracy.
- Collected, isolated, and characterized plant cell and tissue samples for molecular and biochemical studies.
- Operated PCR systems for DNA amplification, including sample preparation, thermal cycling, and result interpretation.
- Performed agarose gel electrophoresis for DNA/RNA analysis and protein separation for molecular profiling.
- Conducted biochemical assays and performed failure analysis on experimental samples.
- Maintained accurate experimental records to ensure data integrity and reproducibility.
- Managed procurement of chemicals, reagents, and laboratory instruments for molecular biology research.
- Supported high-throughput molecular experiments with a focus on accuracy and efficiency.
- Demonstrated strong teamwork, problem-solving ability, and adaptability in high-volume testing environments.
