Vendula

Experimental scientist with 10+ years of hands-on experience designing, executing, and troubleshooting complex flow cytometry experiments across a wide range of biological systems and research questions. Deep expertise in spectral cytometry, high-parametric panel design (40+ parameters), large-scale cell sorting, and high-dimensional data analysis. Experienced working independently on full experimental workflows — from experimental design through data interpretation — often in parallel across multiple projects with competing timelines.

Broad exposure to immunology research contexts including T cell biology, immunophenotyping, primary immunodeficiency, and translational research — gained through both direct bench research and close scientific collaboration on investigator-led projects across Europe. Comfortable operating at the interface of deep technical expertise and scientific problem-solving.

Senior Scientist – Spectral Flow Cytometry at Sony Biotechnology Europe (2018-07 – Present)

European lead expert for spectral flow cytometry with hands-on scientific responsibility across a large and varied experimental portfolio.

• Independently designed and executed complex, high-parametric flow cytometry and sorting experiments across a wide range of biological models and research questions — including T cell biology, immunooncology, infectious disease, and primary immunodeficiency
• Developed and validated high-parametric cytometry panels (40+ parameters) balancing biological signal, spectral complexity, and acquisition efficiency; adapted workflows to diverse sample types and downstream applications
• Designed large-scale screening workflows for high-throughput cell sorting and phenotyping applications, optimizing for throughput, reproducibility, and data quality
• Served as scientific lead on experiments spanning upstream sample preparation through downstream data interpretation, including sequencing-linked workflows and functional assays
• Generated and analyzed large, high-dimensional cytometry datasets using Sony SFA, FlowJo, and OMIQ; applied dimensionality reduction (UMAP, t-SNE) and unsupervised clustering to extract biological insight
• Interpreted complex multiparametric data in the context of experimental hypotheses, supporting downstream decision-making and translational research conclusions
• Operated as the European lead expert for spectral flow cytometry, maintaining hands-on scientific responsibility across a large and varied experimental portfolio
• Ran multiple complex projects simultaneously, managing competing experimental timelines and deliverables independently
• Led a team of 5 application scientists; delivered scientific presentations, technical trainings, and workshops across Europe

Product Specialist – Flow Cytometry Diagnostics at Cytognos S.L. (2016-11 – 2018-07)

Specialized in diagnostic and research flow cytometry workflow design with emphasis on assay robustness, standardization, and reproducibility.

• Designed, optimized, and troubleshot diagnostic and research flow cytometry workflows with emphasis on assay robustness, standardization, and reproducibility
• Developed hands-on expertise in data quality, process control, and assay consistency in high-impact biological datasets

Research Scientist – Immunology at Faculty Hospital Motol (CLIP), Prague (2014-09 – 2015-05)

Development and validation of standardized multi-color flow cytometry assay for primary immunodeficiency patient classification.

• Development and validation of a standardized multi-color flow cytometry assay for classification of primary immunodeficiency patients — full workflow from experimental design to clinical-grade validation
• Co-authored peer-reviewed publication: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410673/

MSc in Immunology – Charles University, Faculty of Science (2014)

BSc in Biology – Charles University, Faculty of Science (2012)