Khizra Bano

Microbiologist with up to 4 years of experience in microbial testing. Proficient in microbial analysis of water, wastewater, and industrial samples with accuracy and adherence to regulatory standards. Detail-oriented and committed to delivering reliable lab results. Seeking to apply microbiological skills in a dynamic and growth-focused role.

Microbiologist at Environmental Services Pakistan (PVT) Ltd. (ESPAK) (2021-10 – Present)

Conducted microbiological analysis of environmental samples and performed microbial detection and enumeration using various validated methods and protocols.

• Conducted microbiological analysis of environmental samples including water, wastewater, soil, and surface swabs.
• Performed microbial detection and enumeration of E. coli, Total & Fecal Coliforms, Pseudomonas aeruginosa, Legionella spp., Streptococci, Enterococci, and fungal organisms using membrane filtration, MPN, and plate count methods.
• Operated and maintained laminar flow cabinets for sterile environments and contamination control.
• Carried out sterility testing and bacterial endotoxin testing using validated protocols and aseptic techniques.
• Prepared and managed ready-to-use culture media and reagents, ensuring proper storage and quality assurance.
• Evaluated and verified commercial microbiological test kits for rapid and confirmatory analysis.
• Performed routine calibration and maintenance of key lab instruments including pH meter, TDS meter, turbidity meter, and conductivity meter.
• Operated and maintained Tuttnauer 3170 ELV vertical autoclave, a fully automatic sterilizer, for sterilization of culture media, lab instruments, and biological waste under validated temperature and pressure cycles.
• Operated various microbiological instruments including Memmert incubator (Model 100-800), compound microscope (Nikon-20), vortex mixer (Bluepard V-20), water bath incubator (Being BWB-12), digital water bath (HH-S4), and mini incubators (Benchmark H2200-HC).
• Operation and calibration of turbidity meter, pH meter, conductivity meter, and TDS meter following standard procedures.
• Supported PNAC audits by maintaining comprehensive documentation and ensuring alignment with ISO/IEC 17025 standards and SOPs.
• Participated in field sampling for water and soil analysis, ensuring sterile sample collection, proper handling of sterile bottles, and adherence to chain-of-custody protocols.
• Provided training and presentations to field and sampling staff on proper sample collection techniques and handling of microbiological containers.
• Ensured strict biosafety compliance in handling and disposal of hazardous and infectious materials.
• Performed Qarshi research international Pvt. Ltd. proficiency testing. Co-ordinated with Chief Chemist in preparation of technical documents required for ISO 17025:2015 accreditation.

B.S. in Biotechnology – University of Management & Technology Lahore (2016 – 2020)

HSSC in Pre-Medical – Govt. College for Women, Gulberg (2013 – 2015)