Sara Chaves

Bioengineer with experience in cell-based assays, molecular biology, and advanced in vitro models, including 3D systems. Skilled in flow cytometry, qPCR, ELISA, and primary human cell culture, with hands-on experience in immune cell characterization and biomarker analysis.

Background in both research and clinical laboratory environments, with strong focus on data quality, reproducibility, and SOP compliance. Seeking roles in biotech, diagnostics, or medical devices.

Laboratory Scientist at University of Minho / i3Bs (2021-01 – Present)

• Isolated and cultured primary human cells, including blood-derived immune cells and skin-derived cells (fibroblasts and keratinocytes)
• Designed and executed cell-based assays using flow cytometry, ELISA, and immunofluorescence for phenotypic and functional characterization
• Developed and optimized 3D in vitro models, enhancing physiological relevance of experimental systems
• Standardized laboratory protocols, contributing to improved reproducibility and experimental consistency
• Analyzed biological datasets and generated figures and reports for publications and project communication
• Collaborated in multidisciplinary teams and supported mentoring of international researchers

Laboratory technician (Clinical Diagnostics) at Unilabs, Porto (2020-09 – 2020-12)

• Performed high-throughput RT-PCR testing for SARS-CoV-2 detection
• Conducted data validation, and quality control to ensure accuracy of diagnostics results
• Followed strict SOPs and regulatory procedures in a clinical laboratory setting
• Maintained sample traceability and documentation aligned with healthcare reporting systems

Research trainee at i3S, University of Porto (2018-02 – 2019-07)

• Cultured primary human cells (endothelial cells and fibroblasts) and cancer cell lines
• Performed qPCR, ELISA, and immunofluorescence-based assays
• Developed and characterized alginate hydrogels (including rheological analysis) for 3D cell culture systems
• Contributed to a peer-reviewed publication

Research trainee at REQUIMTE, Porto (2017-02 – 2018-07)

• Supported analytical workflows in material characterization
• Gained exposure to SEM, XPS, NMR and mass spectroscopy

PhD Candidate in Tissue Engineering, Regenerative Medicine and Stem Cells – University of Minho / i3Bs (2022-03)

MSc in Bioengineering – University of Porto / FEUP (2014-09 – 2019-07)