Sarooj Nadeem

◦ Experience with IPSC/ESC cell line culture.

◦ Performed data generation, collection, documentation, analysis and presentation. ◦ Prepared variety of different written communications, reports and documents.

◦ Worked within applicable standards, policies, and regulatory guidelines to promote safe working environment.

◦ Evaluated and optimized beta cell differentiation protocol using 2D/3D culture method.

◦ Designed and performed standard molecular biology assays.

◦ Analyze sequencing data – Sanger and NGS. Troubleshoot got data if necessary ◦ Performed Flow cytometry analysis on differentiated beta like cells from hIPSCs

◦ Conducted Confocal microscopic imaging on IPSCs

◦ Fluorescent Microscopy

◦ Flow cytometry

◦ ELISA, Cloning, q/PCR

◦ Western Blotting, plasmid purification, cloning, agarose and polyacrylamide gel electrophoresis and restriction digests

◦ Sanger DNA Sequencing

◦ Performed data generation, collection, documentation, analysis and presentation.

Research project: Investigating the role of serotonergic regulation in diabetes type II and proliferation of hIPSCs derived pancreatic beta cell

Techniques Used

Maintaining Stem cell culture, Gene cloning, Confocal microscopy, Flow cytometry, ICC/IHC/IFC

Cloning/plasmid construction and qPCR

Organizing lab meetings

Maintaining cell lines

MASTER'S THESIS RESEARCH – CENTER OF EXCELLENCE IN MOLECULAR BIOLOGY, UNIVERSITY OF THE PUNJAB, PAKISTAN

◦ Research project " SNP association studies of Chronic Obstructive Pulmonary disorder in Pakistani population by genotyping"

◦ Cloning, qPCR and DNA quantification, mini sequencing. ◦ Supervisor: Dr. Marium Shahid