Cytogenetics Technologist - Quest Diagnostics/Medfusion Labs
(2024-01)
- Examine chromosomes found in biological specimens to detect abnormalities.
- Perform chromosome analysis of cancer bone morrow specimens for abnormalities.
- Assist in training new techs on chromosome analysis.
Senior Molecular Technologist - Labcorp
(2022-10 - 2024-12)
Proficient in PCR, RT-PCR, Western blotting, FragileX assay, MLPA assays, AGG, methylation PCR assay, loading PCR plates using capillary electrophoresis. Experienced in interpreting and reporting Fragile X result analysis. Strong knowledge of laboratory quality control and quality assurance protocols.
Skilled in maintaining and troubleshooting molecular testing equipment. Ability to train and mentor junior technologists when requested. Excellent attention to detail and accuracy in documentation and reporting.
Effective communication and collaboration skills in a team environment. Strong organizational and time management abilities.
- FISH Analysis on Prenatal Samples: Conducted Fluorescence In Situ Hybridization (FISH) assays on prenatal samples, including chorionic villus sampling (CVS) and amniotic fluid, to detect chromosomal abnormalities and assess fetal genetic health.
- DNA Extraction: Performed extraction of DNA from peripheral blood and prenatal samples (CVS and amniotic fluid) using standard protocols for high-purity genomic DNA suitable for downstream molecular analysis.
- Fragile X Syndrome Assay: Carried out specialized testing for Fragile X syndrome, utilizing PCR and Southern blot techniques to assess CGG repeat expansions and methylation patterns associated with the condition.
- AGG Repeat Assay: Executed AGG repeat analysis to study the distribution of AGG interruptions in the Fragile X region, providing insight into the genetic factors contributing to the syndrome.
- Methylation PCR Assay: Performed Methylation-Specific PCR (MS-PCR) assays to evaluate the methylation status of specific gene regions, crucial for conditions like Prader-Willi and Angelman syndromes.
- MLPA (Multiplex Ligation-dependent Probe Amplification): Conducted MLPA assays to detect copy number variations (CNVs) and gene deletions/duplications, enhancing diagnostic capabilities for genetic disorders like DMD and others.
- DMD Assay (Duchenne Muscular Dystrophy): Conducted genetic analysis for Duchenne Muscular Dystrophy (DMD) using PCR-based assays to identify deletions or duplications in the dystrophin gene.
- Sanger Sequencing: Performed Sanger sequencing for mutation analysis in targeted genes, providing definitive genetic diagnoses and helping to validate results from other screening techniques.
Senior Cytogenetic Technologist - Labcorp
(2019-07 - 2022-09)
- Perform analysis of several types of patient specimens labeled with fluorescent probes by using various microscopes and computerized imaging equipment.
- Training of new and seasoned technologists in preparation of patient samples, assay analysis and reporting of FISH results.
MLT - BLOT - Oxford Immunotec
(2018-04 - 2019-07)
Mainly working on Western blotting. Running blots on live serum samples using autoblot machines. Worked in a cGMP environment.
Cytogenetics Technologist - Bio-reference Laboratory/OPKO
(2005 - 2017-09)
Worked over 12 years as a cytogenetics technologist in Bio-reference labs/Opko health inc. As a cytogenetic technologist handled live specimens, gained in-depth knowledge on the various areas.
- Examined chromosomes found in biological specimens to detect abnormalities.
- Harvested cell cultures using substances such as mitotic arrestant, cell releasing agents, and cell fixatives.
- Preparation and storage of media to maintain potential of hydrogen (pH), sterility, or ability to support growth.
- Prepared slides of cell cultures following standard procedures.
- Determined optimal time sequences and methods for manual cell harvests.
- Hands-on experience in conducting cytogenetic testing, analysis and documentation in a laboratory setting.
- Performed maintenance on laboratory equipment. Provided written documentation of laboratory work and maintained accurate records.
- Immense knowledge of cytogenetic fundamentals, procedures.
- Operational knowledge of laboratory apparatus, cytologic equipment, Microsoft Office, and internet service.
- Familiarity with data interpretation, sterile techniques, and molecular cytogenetics.
- Ability to conduct and complete all assigned tests, in a timely manner.
- Ability to communicate with all employees of the institution, in a positive manner.
- Familiarity with all cytogenetic techniques and executed standard karyotyping activities.
- Documented and maintained sample information, patient data, and cytogenetics images.
- Operated, calibrated, and maintained all assigned laboratory equipment.
- Cleaned laboratory premises, maintained inventory of supplies and disposed of medical waste safely.
Research Assistant - UMASS Hospital
(2003 - 2004)
Worked as a research associate in the Microbiology/Molecular biology department. Mainly worked on Western Blotting technique, southern blotting, Agarose gel electrophoresis, polyacrylamide gel electrophoresis, Cloning, Transformation, Rapid nuclear extraction from tissues and cell suspensions and calculating protein concentration, PCR technique, RT-PCR, preparation of competent E.coli cells, transformation of yeast cells & E.coli cells, minipreps of DNA, ELISA, preparation of media, culture. Developed expertise in western blotting technique.
Maintaining records for results. Performed maintenance on laboratory equipment. Provided written documentation of laboratory work and maintained accurate records.
