Flow Cytometry Specialist - Spark Therapeutics - Philadelphia, PA
(2019-10 - 2021-00)
- Performed cell isolation from in vitro and in vivo sources and examined cell phenotypes and in some cases cell function.
- Collected data and summarized data and interpreted the data and provided conclusions and presented research to group
- Worked under strict GLP guidelines. Authored SOPs.
- Processed clinical samples according to SOPs under strict GLP guidelines examining patient immune response to the experimental virus.
VWR Contractor, Associate Scientist - Johnson & Johnson - Spring House, PA
(2018-07 - 2019-00)
- Performed tissue culture, ELISAs, westerns and flow cytometry to support immuno-oncology project
Senior Biologist - ELI Lilly - Indianapolis, IN
(1997-11 - 2017-04)
Used molecular, cellular and ex vivo approaches to assess candidate protein and small molecule drugs for autoimmunity, cancer, diabetes, inflammation, and pain for advancement to phase I.
- Adept at designing, validating and executing many immunological techniques such as ELISAs, ELISpot, immunomagnetic cell separation, immunoassays, cell-based assays, Acumen imaging, Luminex, LICOR, MLR, MSD, proliferation, cytotoxicity and reporter assays.
- Adept at designing, validating and executing molecular techniques such as cloning, various transfection protocols including retroviral, protein expression and characterization. Proficient at PCR, DNA and RNA isolation from cells and tissue, qPCR, Quantigene, RNA silencing and DNA sequencing, Sequencher, Vector NTI. Transient and stable cell line development.
- Adept at tissue culture including cell line and primary lines. Skilled at isolating and culturing of T and B cells, immature and mature dendritic cells, and macrophage cultivation. Experienced culturing and differentiating stem cells into mast cells.
- Experienced in animal handling, injections, genotyping and models.
- Skilled in the use of flow cytometer including Beckman-Coulter FC500 and BD Fortessa. Proficient at bulk and single cell sorting using FACSARIA II using an array of different cell types.
- Served as group contact for FACS assistance and troubleshooting. Instructed others in FACS techniques and use of the instrumentation and performed maintenance and calibration. Worked with technicians on repair of units.
- Isolated and cultured bacteria from animal samples for identification and antibiotic susceptibility assays.
- Performed in vitro bacterial invasion and killing assays.
- Performed in vivo bacterial infections in diary cattle and swine to assess efficacy of therapeutics.
- Proficient at laboratory equipment such as plate readers, fluorimeters, tissue processors, UV spectroscopy, HPLC and FLIPR. Radioactivity use and counters.
