Edwin Ragwan

Undergraduate instruction in biochemistry and general chemistry labs, and graduate instruction in advanced protein structure-function discourse with strong emphasis on inclusive and evidence-based pedagogy. Protein biochemistry and enzymology; focus on structure-guided functional analysis with expertise in structural biology, kinetic analysis, molecular biology, degradation via the Ubiquitin Proteasome System

Chemistry Faculty (Postdoctoral Scholar) - Villanova University - Villanova, PA

(2022-01)

• Taught 1st and 2nd semester upper-level biochemistry laboratory courses
• Taught Graduate lecture course on Advanced Protein Biochemistry
• Taught General Chemistry I laboratory
• Facilitated Professional Development Seminar course
• Oversaw Teaching Assistants for laboratory courses
• Mentored undergraduates in the Kraut Research lab

Protein Biochemistry Researcher - Villanova University - Villanova, PA

(2022-01)

Conduct protein biochemistry research on eukaryotic proteasome looking at mechanisms of substrate unfolding and degradation; experiments try to explain why some protein substrates are prematurely released, resulting partially degraded proteins can sometimes cause cell toxicity and may be linked to neurodegenerative disease

• Design and perform degradation kinetic assays with novel substrate models
• Utilize molecular cloning, substrate expression and purification, substrate labeling, degradation kinetic assays, CRISPR-Cas9 gene editing
• Supervise undergraduate researchers

Graduate Research Mentor - Bryn Mawr College - Bryn Mawr, PA

(2016-01 - 2022-05)

• Help oversee the projects of various undergraduate students
• Taught new techniques, interpreting data, and designing experiments

Protein Biochemistry Researcher - Bryn Mawr College - Bryn Mawr, PA

(2016-01 - 2022-05)

Protein biochemistry involving structural characterization and modification of proteins in the mevalonate pathway

• Design of novel HMG-CoA reductase proteins that show altered cofactor specificity
• Utilized techniques including mutational analysis, protein expression and purification, kinetic analysis, and X-ray crystallography

Graduate Teaching Assistant - Bryn Mawr College - Bryn Mawr, PA

(2016-01 - 2021-12)

• Assisted in technique and grading of laboratories in General Chemistry I, and Organic Chemistry I&II

Researcher - University of Pennsylvania - Philadelphia, PA

(2013-04 - 2014-02)

Worked with postdoctoral researcher in the Weiner Lab exploring DNA vaccines targeting Respiratory Syncytial Virus and botulinum neurotoxin

• Investigated cell-based and mice-models, utilizing techniques such as mammalian cell-culture, ELISA and ELISPOT experiments

Undergraduate Co-op - Janssen R&D, Biotechnology Center of Excellence, Johnson & Johnson Pharmaceutical R&D - Radnor, PA

(2011-05 - 2011-12)

Investigating the quantity and distribution of neonatal Fc receptor transcripts and protein in various segments of human, non-human primate, and rodent gut as a candidate for drug transport

• Utilized RNA extraction, RT-PCR, ELISA, Western Blot, mammalian cell culture, mammalian transfection and fluorescence

Math Tutor - Mathnasium - Pennsylvania, USA

(2012-01 - 2015-12)

• Taught remedial and prescriptive math concepts for K-12 students

Teaching Assistant - Eastern University - St. Davids, PA

(2009-01 - 2012-12)

• Served as a TA for biology courses

Researcher - Eastern University - St. Davids, PA

(2009-06 - 2009-12)

Isolation and analysis of proteins from the plant pathogen Erwinia amylovora

• Utilized expression and purification of protein techniques and product analysis using HPLC

Ph.D. - Chemistry (Biochemistry) - Bryn Mawr College (2018-09 - 2022-05)

M.A. - Chemistry (Biochemistry) - Bryn Mawr College (2016-09 - 2018-05)

B.S. - Biochemistry - Eastern University (2008-09 - 2012-05)