Jamie r. Burke
Laboratory Technician IV
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About
ASCP Hematology and Chemistry certification eligible. Performed high-complexity clinical laboratory assays in a fast-paced hospital environment, including specimen processing, quality control, data entry, and troubleshooting instrumentation. Maintained compliance with CLIA, CAP, and OSHA standards while collaborating with multidisciplinary teams to support patient care.
Demonstrated competencies on all laboratory protocols, emphasizing accuracy, safety, and best practices in biospecimen handling.
Experience with Roche COBAS, Sysmex, Cepheid, Clinitek Advantus analyzers.
Experience
Laboratory Technician IV - CLEVELAND CLINIC - Medina, OH
(2022-10 - 2025-05)
ASCP Hematology and Chemistry certification eligible. Performed high-complexity clinical laboratory assays in a fast-paced hospital environment, including specimen processing, quality control, data entry, and troubleshooting instrumentation. Maintained compliance with CLIA, CAP, and OSHA standards while collaborating with multidisciplinary teams to support patient care. Demonstrated competencies on all laboratory protocols, emphasizing accuracy, safety, and best practices in biospecimen handling.
Experience with Roche COBAS, Sysmex, Cepheid, Clinitek Advantus analyzers.
Academic Laboratory Manager - KENT STATE UNIVERSITY - North Canton, OH
(2021-08 - 2022-09)
Coordinated space and equipment requirements for eight college-level academic laboratories, including maintaining consumable stocks, working solutions, facility safety equipment, and individual teaching lab supplies such as cell culture preparation, anatomy and physiology dissection preparation, and organic/general chemistry preparation. Provided guidance to leadership regarding purchasing patterns, capital equipment contracts, and compliance with environmental health and safety standards, including biohazard regulated waste disposal and hazardous waste containment and removal.
CRO - Clinical Laboratory Assistant, Immunological Assays - C.T.L - Shaker Heights, OH
(2019-10 - 2020-01)
Patient Access Liaison I - Summa Health System - Akron, OH
(2018-04 - 2018-09)
Adjunct Professor, Anatomy and Physiology - Stark State College - Akron, OH
(2018-08 - 2018-12)
Sr. Research Technologist - Cleveland Clinic/LRI - Cleveland, OH
(2016-01 - 2016-12)
AP/IB Biology Teacher - Akron Public Schools - Akron, OH
(2015-01 - 2016-12)
Research Coordinator II - NORTHEAST OHIO MEDICAL UNIVERSITY - Rootstown, OH
(2011-06 - 2015-09)
- Provided research support to 5 PIs in several study areas: oncology, metabolic/cardiovascular and neurodegenerative disease.
- Designed and executed biochemical/cell-based screening assays of drugs and novel compounds: dose-response, time-course, enzyme binding and cell proliferation studies.
- Adopted protocols for signaling pathway identification by cloning and gene silencing, luciferase gene reporter assays, qRT-PCR, and protein analysis via Flow, ELISA, Westerns
- Supervised technicians and students ensuring compliance with timelines, quality, and institutional safety.
- Coordinated Pharmaceutical Sciences capital equipment procurement, installation and verified operating specifications.
- Coordinated study sample testing and storage per IRB approved protocol guidelines; maintained document control and database of records per institutional guidelines.
- Supervised scientific equipment maintenance, established service contracts with external agencies, created SOPs for department to maintain quality and reduce costs.
Sr. Research Technologist - AKRON CHILDREN'S HOSPITAL - Akron, OH
(2005-02 - 2011-01)
Performed experimental research to elucidate molecular mechanisms underlying pediatric cancer. Designed, executed, and analyzed experiments using a range of advanced techniques including:
- PCR, qPCR
- RNA, DNA, and protein extraction
- Electrophoresis; western, southern, and dot blotting; ELISA
- Antibody selection and optimization
- Methylation and restriction enzyme analysis; COBRA assay
- Gel and film densitometry; ECL imaging and optimization
- Recombinant DNA methods: transient and stable transfections
- Culture identification, aseptic technique; experience with mesenchymal stem cells and various immortalized cell lines
- Immunocytochemistry and immunofluorescence
- Cloning; transient and stable transfections
- Soft agar colony formation, apoptosis, and MTT assays
- Cell banking, documentation, staining, and imaging
- Confocal microscopy
Sr. Research Assistant - NORTHEAST OHIO MEDICAL UNIVERSITY - Rootstown, OH
(2004-01 - 2005-12)
Gained experience in biotech project management by participating in design meetings with key stakeholders. Researched and reported on FDA clinical device regulations and EPA water quality studies. Performed patent searches, summarized technical specifications. Conducted purchasing and inventory control; ensured reporting compliance with Federal and institutional guidelines, compiled post-award expenses indirect/direct costs and capital expenses for Grants Accounting.
Research Associate - UNIVERSITY SPACE RESEARCH ASSOCIATION NASA Glen Research Center - Brook Park, OH
(2002-01 - 2004-12)
Grant funded research support - made significant contributions to project by establishing in vitro bone cell model. Adopted Osdoby et al Osteoclastogenic model utilizing macrophage cell line RAW.264.7 induced with RANKL and grown on a mineralized matrix. Demonstrated resorption of matrix via TRAP staining.
Education
Master of Science - Biology - University of Nebraska, Kearney (2010-07)
Bachelor of Science - Biology - Kent State University (2000-01)
