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Jhanavi Sabharwal

Jhanavi Sabharwal

Undergraduate Research Assistant

Talent
TampaMember since September 22, 2026
RNA extractionPrimer designGene-expression analysisTranscription analysisExperimental designMammalian cell cultureHEK cell maintenanceAseptic techniqueCell passagingImmunohistochemistryCD8 stainingImmunofluorescenceParaffin embeddingFluorescence microscopyMurine modelsLymphocyte survival experimentsDissectionsBioluminescence imagingSPSSExcelRayyanfMRI data analysis in SPM
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RNA extractionPrimer designGene-expression analysisTranscription analysisExperimental design

About

Maintain and passage HEK cell cultures using sterile tissue culture and aseptic techniques. Perform paraffin embedding and processing of human tonsil tissue samples. Conduct CD8 immunohistochemical staining and assist with immunofluorescence workflows.

Experience

Undergraduate Research Assistant at USF Health Morsani College of Medicine – HIV Research Laboratory (2024-03 – 2026-05)

  • Maintain and passage HEK cell cultures using sterile tissue culture and aseptic techniques.
  • Perform paraffin embedding and processing of human tonsil tissue samples.
  • Conduct CD8 immunohistochemical staining and assist with immunofluorescence workflows.
  • Perform cell counting and fluorescence imaging using Echo Revolution microscopy systems.
  • Organize tissue-slide databases and maintain experimental records.

Research Assistant at Sleep Research Laboratory (Dr. Christina McCrae) (2026-01 – Present)

  • Conduct SPSS analyses on clinical datasets from the SPINII pain and insomnia study.
  • Assist with fMRI data processing and neuroimaging workflows.
  • Participate in home visits administering NIH Toolbox assessments and psychological surveys.
  • Contribute to research investigating sleep interventions, pain outcomes, and opioid reduction.
  • First author abstract submitted to the Society of Behavioral Sleep Medicine.

Research Intern at Moffitt Cancer Center – Mathematical Oncology Department (2025-03 – 2025-09)

  • Assist with murine mammary cancer research and anesthesia-based experimental protocols.
  • Gain experience with live murine models, dissections, tissue handling, and bioluminescence imaging.
  • Conducted data analysis on anesthesia research project independently.

Research Assistant at Spartina Gene Expression Project

  • Performed RNA extraction from Spartina samples using liquid nitrogen tissue homogenization.
  • Prepared biological samples for downstream gene-expression analyses.
  • Contributed to manuscript preparation examining transcriptional responses to environmental stressors.
  • Expected middle-author manuscript submission (2026).

Research Assistant at Advanced Genetics & Cell Biology Research Laboratory (2025-08 – 2025-12)

  • Designed mRNA primers for gene-expression studies.
  • Conducted transcription-level analyses of cellular responses.
  • Investigated dose-dependent effects of saccharin exposure on murine lymphocyte survival.
  • Applied molecular biology techniques to evaluate gene-regulatory mechanisms.

Emergency Medical Responder at USF Student Health

250+ hours

Health Support Staff at USF Student Health

90+ hours

Clinical Volunteer at AdventHealth

Emergency Department and Respiratory Unit

Education

B.S. in Biology (Medical Concentration), Minor in Biomedical Physics – University of South Florida (2026-05)

Skills

Undergraduate Research Assistant

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