Kalash Neupane

PhD Candidate in Regulatory Biology with expertise in oncology, immunology, inflammation, cardiovascular disease, and translational research. Experienced in designing and executing preclinical studies using urethane-induced and Lewis Lung Carcinoma (LLC) mouse models, human lung cancer cell lines, microbiome sequencing, immunohistochemistry (IHC), immunofluorescence (IF), multiplex cytokine analysis using meso-scale Discovery (MSD), and molecular biology techniques. Co-author of multiple peer-reviewed publications in high-impact journals.

Proven track record in therapeutic evaluation, biomarker discovery, tumor microenvironment characterization, and target validation supporting drug discovery and development.

Graduate Research Assistant - Cleveland State University - Cleveland, OH

(2021-08)

• Lead investigator on projects evaluating inflammatory mechanisms driving lung cancer progression and cardiovascular disease.
• Designed and executed studies utilizing urethane-induced lung cancer models and Lewis Lung Carcinoma (LLC) mouse models for therapeutic evaluation and mechanistic investigations.
• Led microbiome sequencing studies investigating links between microbial communities, inflammation, and cancer progression.
• Performed multiplex cytokine and chemokine analysis using Meso Scale Discovery (MSD) technology.
• Characterized tumor microenvironments using Immunohistochemistry (IHC) and Immunofluorescence (IF) to evaluate T-cell and macrophage infiltration and localization.
• Conducted TUNEL assays to assess apoptosis in tumor tissues.
• Performed drug screening and therapeutic evaluation using human lung cancer cell lines and preclinical mouse models.
• Applied molecular biology techniques including qRT-PCR, PCR, Western blotting, ELISA, DNA/RNA isolation, protein analysis, and tissue characterization.
• Utilized R and Python for statistical analysis, pathway analysis, and microbiome data interpretation.
• Collaborated with multidisciplinary research teams to identify therapeutic targets and translational biomarkers.

Teaching Assistant - Cleveland State University - Cleveland, OH

(2021-08)

• Taught undergraduate and graduate laboratory courses in biology and microbiology.
• Mentored students in laboratory techniques, scientific communication, and experimental design.

PhD - Regulatory Biology - Cleveland State University (2026-12)

Master of Science - Medical Microbiology - Tribhuvan University

Bachelor of Science - Microbiology - Tribhuvan University