Letealia Oliver

Scientific Research experience within multiple research areas including Cell Biology, Vaccine development, Pediatric Genetics, Drug Metabolism and HIV Research.

Size Exclusion

Chromatography, Flow Cytometry, Data Analysis, Cell Culture, Animal Surgery, Electrophoresis, training key personnel, Immunology and Vaccine development

Research Analyst - Duke University Medical Center - Durham, NC

(2006-10)

• Routinely prepare cells and blood from various animal models for Immunophenotypic analysis using antibody panels to characterize B and T cell populations
• Cell Sorting and analysis of flow data using FlowJo software
• Manage cryopreservation of cells and maintain accurate records in a cell inventory database (FreezerPro)
• Regularly perform Calcium Flux studies with fluor-4 kit
• Accurately record current antibody supply to ensure availability for use
• Routinely carry out antibody and hook titrations with Ramos cell lines
• Provide weekly summary of work activities
• Routinely check hook inventory for mouse experiments
• Conduct Fine Needle Aspirate and bone marrow collections with Ficoll Gradient to evaluate the immune response in Non-Human Primates
• Studies involving the immune response in T cells via Intracellular Cytokine Staining Assays (ICS) and Activation Induced Marker (AIM) Assays
• Routinely train emerging scientists in the processes of immunophenotypic assays and data analysis via the FlowJo software
• Experience with using B cell enrichment studies using Robosep
• Key personnel for maintaining and ordering ab supplies
• Carry out data management, instrument startup and quality control on BD analyzers and sorters
• Fully comply with appropriate DHVI/GHRB Safety and compliance SOP responsibilities
• Daily operate 2-3 FPLC s to purify proteins for internal and external customers for DHVI
• Proteins ranged from small V1V2tags proteins to gp120s, gp140s, IgGs and IgAs
• Coordinate with SPR QC and Gel QC partners to pull together data for finished QC reports for HIV envelope distribution
• Successful training of others on purification platforms via written SOPs
• Performed routine maintenance and troubleshooting techniques to keep instruments operational and entering service requests as needed with minimal downtime
• Directly involved in training others to run FPLC's and follow methods outlined in Standard Operating Procedures
• Provided continued assistance to others with fraction selection and instrument issues
• Method Development for new column platforms
• Weekly run QC's on all SOSIP Trimers produced and log results in power point format
• Routinely, provided daily Purification Plans and Weekly summaries of work
• Executed monthly cleaning schedule for FPLC's and storage of columns in Sodium Azide at the end of each week
• Independently organized and executed daily tasks to ensure efficiency and productivity
• Routine cell sorting and analytical profiling over multiple platforms including BD FACS ARIAII cell sorters and BD LSRII analyzers
• Regular maintenance and repair of instruments
• Assist investigators in troubleshooting experiments and data collection
• Maintained records to ensure all data is captured for final reports
• Instrument startup QC and maintenance
• Monthly billing of instrument time and usage
• Routinely working at BSL2 and BSL3 levels
• Select Agent training to perform research involving select agents

Master of Science - Biology - North Carolina Central University (1989-09 - 1991-06)