Research Associate (USA) at NEW JERSEY INSTITUTE OF TECHNOLOGY (2026-01 – Present)
Developed an online ultrafast microdroplet digestion method for rapid ADC structural characterization and antibody charge variant analysis
- Characterized ADC drug-mimic using IdeS, PNGaseF, and Endo S2 enzymatic workflows integrated with microdroplet-accelerated digestion, followed by pseudo-DAR calculation.
- Devised a strong cation exchange-based chromatographic method for the separation of NIST mAb charge variants and performed ultrafast digestion in microseconds based on our online microdroplet digestion technique using enzymes such as IdeS and FabRICATOR Xtra.
Doctoral Research Assistant at NEW JERSEY INSTITUTE OF TECHNOLOGY (2021-09 – 2025-12)
- Developed a standard-free absolute quantitation method for post-translationally modified peptides and mAb
- Implemented derivatization techniques to incorporate electrochemical tags to phosphopeptides and glycopeptides.
- Quantified glycopeptides from tryptic mAb digest. HILIC separation was used to separate glycopeptides.
- Achieved cost-effective & standard-less absolute quantification.
- Developed a standard-free absolute quantitation method to prove protein 3D conformational structure
- Designed an electrochemistry-assisted mass spectrometric method (CMS) to predict protein 3D structure as an alternative technique to X-ray crystallography.
- Labelled Lysine and Tyrosine residues of proteins with electroactive functional groups, such as Fe-C6-NHS and 4-nitrobenzene diazonium, at native pH, and quantified digested peptides by CMS technique.
- Validated the CMS method by correlating labeling results with solvent-accessible surface area (SASA) values derived from X-ray crystallographic structures.
- Developed a quantitative mass spectrometry imaging technique using desorption electrospray ionization combined with coulometric mass spectrometry (CMS) techniques
- Optimized RP-LC–HRMS workflows to separate olanzapine and metabolite mixtures and achieve fmol-level sensitivity in tissue.
- Enabled calibration-free absolute quantification of drug metabolites without costly, time-consuming synthesis of reference standards.
- Developed a rapid ionization method for the analysis of PFAS
- Designed a novel paper spray–based MS ionization technique that simultaneously desalted and enriched samples for trace-level (ppt) identification and quantification of PFAS across multiple matrices.
- Optimized spray ionization parameters (solvent composition, spray voltage, and emitter distance) to maximize ionization efficiency and analytical sensitivity.
Pharmaceutical Quality Control Officer at Advance Chemical Industry (ACI) (2014-09 – 2018-07)
- Performed GMP-compliant QC assays, impurity profiling, and stability testing of APIs and finished dosage forms.
- Executed analytical testing using HPLC-UV, GC, FT-IR, UV-Vis, AAS, and Karl Fischer titration.
- Ensured compliance with USP, BP, and ICH guidelines through adherence to SOPs, GMP, ALCOA+, and accurate documentation practices.
