Muhammad Jamal

PhD-trained molecular biologist with 8+ years of research experience in molecular biology, cell biology, genomics, cancer research, and preclinical model systems. Experienced in mammalian cell culture, molecular assays, nucleic acid extraction, assay development, transcriptomic analysis, CRISPR/Cas9 technologies, and animal models. Proven ability to design experiments, optimize workflows, troubleshoot technical challenges, analyze complex datasets, and collaborate effectively across multidisciplinary teams.

Experienced working in U.S. research environments and currently authorized to work permanently in the United States without sponsorship.

Research Scientist (Postdoctoral Research Associate) - University of Nebraska Medical Center (UNMC) - Omaha, NE

(2024-10)

• Lead translational cancer research projects focused on therapeutic target validation in pancreatic cancer.
• Perform mammalian cell culture and maintain multiple human and mouse cancer cell lines using aseptic techniques.
• Develop and execute cell-based functional assays including proliferation, apoptosis, viability, migration, invasion, colony formation, and drug sensitivity studies.
• Perform molecular analyses including RNA isolation, RT-qPCR, Western blotting, immunofluorescence, and immunohistochemistry.
• Generate genetically modified cell models using CRISPR/Cas9 and lentiviral approaches.
• Conduct in vivo mouse studies and support tissue collection, processing, and downstream molecular analyses.
• Analyze experimental data and prepare reports, presentations, manuscripts, and grant-related materials.
• Maintain detailed laboratory records and collaborate with multidisciplinary teams to achieve project objectives.

Research Assistant (PhD Research) - Wuhan University - China

(2019 - 2024)

• Investigated molecular mechanisms driving T-cell acute lymphoblastic leukemia progression and therapeutic resistance.
• Maintained mammalian cell cultures and performed functional assays including proliferation, apoptosis, migration, invasion, and drug response studies.
• Developed stable gene overexpression and knockdown models for pathway analysis and target validation.
• Performed RT-qPCR, Western blotting, immunohistochemistry, immunofluorescence, and transcriptomic analyses.
• Established and characterized xenograft mouse models to evaluate tumor progression and therapeutic responses.
• Integrated molecular, cellular, and in vivo datasets to identify biomarkers associated with disease progression.
• Contributed to scientific publications, presentations, and technical reports.

Research Assistant (Genomics & Molecular Biology) - King Abdullah University of Science and Technology (KAUST) - Saudi Arabia

(2021 - 2023)

• Performed RNA-seq, ChIP-seq, Hi-C, and SPRITE library preparation workflows and downstream genomic analyses.
• Conducted nucleic acid extraction, sample processing, quality control assessment, and sequencing preparation.
• Performed immunoprecipitation and peptide pull-down assays for protein interaction studies.
• Processed and interpreted large-scale genomic and transcriptomic datasets.
• Optimized experimental procedures and troubleshooting strategies to improve reproducibility and data quality.

Research Assistant – CRISPR & Functional Genomics - Huazhong Agricultural University - China

(2014 - 2017)

• Developed endogenous Type III-A CRISPR-Cas systems for genome editing and RNA interference in Mycobacterium tuberculosis.
• Conducted genome-wide CRISPRi studies to identify essential genes and regulatory pathways.
• Performed molecular cloning, nucleic acid extraction, PCR, bacterial culture, sequencing validation, and functional characterization.
• Designed guide RNAs and optimized genome engineering workflows.

PhD - Immunology - Wuhan University

MS - Preventive Veterinary Medicine (Microbial Genetics) - Huazhong Agricultural University

BS (Hons) - Biotechnology - University of Malakand