Ojaswee Dahal

Highly skilled and dedicated scientist with about 10 years of experience in a biotech company located at Gaithersburg, MD. As an active team member, I have been providing my services to the company in performing the time-bound research projects pertaining to cloning, sequencing, BAC library construction and recombinant antibody technology. I have been actively involved in the isolation of DNA and RNA, restriction enzyme digestion, ligation, transformation, and screening of positive clones.

My involvement in phage display research involves growing and harvesting of phages, titer determination by plaque assay, bio panning, Elisa and phage DNA isolation for cloning and sequencing. Cell culture responsibilities include maintenance of primary and transformed cell lines, transfection, expression evaluation and protein purification.

Scientist I - Seracare (LGC)

(2022)

Direct and supervise the day-to-day activities of the R&D department. Implement operational policies and procedures to ensure and accuracy of workflows. Use 6s to ensure meeting project goal deadlines and performance.

• CELL CULTURE: Proficient in Mammalian Cell culture, lymphoma cell lines, Retina cell lines AML cell line HK293T. Specializing in Adherent and supervision Tumor cell Lines.
• Conducted cell Transfection and nucleofection and analyzed the GFP expression in a fluorescence microscope
• Maintained the Flow cytometer, worked in a BSC-III LAB Setting
• Expert in nucleic acid analysis, encompassing Genomic DNA, RNA, VIRAL RNA, DNA extraction utilizing Qubit/Nanodrop/Agilent Real-time PCR, droplet digital PCR and Bioanalyzer
• Cloning vector with the gene of interest. CRISPER WITH CAS12. Site directed mutagenesis, amplification of DNA based on the designed project
• Project 1: Quantitative FFPE Blocks
• Project 2: Lentiviral (cloning with vector, make Midi prep or Maxiprep DNA, transfection with HEK293T cells, Transduction with mammalian cells, AML cell line, RPE-1 cell line make gDNA, ddpcr: quntasoft1.7.4)
• Project 3: AML
• Project 4: Brca1 and Brca2 mutations cloned in targeting vector with addition deletions, Transfected that mutations into the GM cells. Likewise CYP2D6 BACS MUTATIONS cloned, make recombineering site, adding plasmid mutations to that site. Analyze the data by digital pcr
• Flow cytometer: preparation of samples, maintenance of equipment (Beckman coulter)
• Lab maintenance: Decontamination incubator, laminar hood, water bath calibration
• AACR PROJECT lab work presentation

Scientist I - Rx Biosciences LLC

(2014 - 2022)

• Genomics: Isolation of nucleic acids including plasmid DNA, RNA, genomic DNA from cells, animal tissues and plants
• High throughput DNA isolation from BAC and Fosmid library clones
• Large scale plasmid DNA isolation including maxi, midi and mega-preps
• Set up primers, PCR and RT PCR
• Purification of DNA from gel including the PCR products
• Setting of restriction enzyme digestion reactions
• Native and denaturing DNA and RNA agarose gels
• Cloning of DNA in various sequencing and expression vectors
• Proteomics: Preparation of cell lysates after transfection, and or induction of mammalian, E. coli and yeast cells
• Protein purification using metal affinity and protein A/G resins
• Protein concentration estimation by Spectrophotometric methods
• Native and denaturing SDS-PAGE
• Western Blot Analysis
• Protein concentration and preservation
• HPLC (received hands-on training)
• Covid-19 testing (under CLIA)
• Make DNA, RNA and RT-PCR from covid-19
• Phage Display Techniques: Maintenance of antibody libraries
• Titration and Bio panning
• Phage rescue and validation of positive clones by sequencing and ELISA
• Cell Biology: Maintenance of transformed and primary adherent and suspension mammalian cells
• Transient Transfection
• Preparation of cell lysates for protein purification, western blotting and/or immune-precipitation

Research Assistant III - Rx Biosciences LLC

(2009 - 2014)

Lab Technician - Rx Biosciences LLC

(2009 - 2014)

Master of Science - Tribhuvan University Nepal

Bachelor of Science - Life Sciences - Tribhuvan University Nepal

Dental Assistant - Montgomery College (2008 - 2008) Wheaton, MD