Oscar Rodriguez

Biochemistry research professional with post-baccalaureate research experience in protein expression, purification, characterization, and assay development. Experienced with recombinant protein production, chromatography, ELISA, yeast-display screening, and biochemical techniques in a research laboratory setting. Seeking opportunities in academic research or the biotechnology/biopharmaceutical industry where I can apply my laboratory expertise, analytical skills, and experience developing and optimizing experimental workflows.

Post-Baccalaureate Research Assistant - Battelle Memorial Institute - Richland, WA

(2024-02 - 2026-08)

• Conducted research involving recombinant protein expression, purification, characterization, and biochemical analysis.
• Expressed recombinant proteins in E. coli, including BL21(DE3), using IPTG induction and optimized growth and expression conditions.
• Performed protein purification using Ni-NTA affinity chromatography, HisTrap columns, ion-exchange chromatography, and size-exclusion chromatography.
• Operated ÄKTA chromatography systems and prepared, optimized, and evaluated chromatography methods for protein purification.
• Performed protein concentration and buffer-exchange procedures using Amicon Ultra centrifugal filters and PD-10 desalting columns.
• Prepared and optimized protein buffers involving Tris, NaCl, imidazole, glycerol, and reducing agents such as TCEP.
• Troubleshot protein solubility, aggregation, precipitation, concentration, and purification challenges.
• Performed biochemical assays and protein characterization, including ELISA and spectrophotometric analysis.
• Worked with protein labeling techniques using fluorescent dyes including Alexa Fluor 647 and FITC.
• Conducted research involving nanobody discovery and screening using yeast surface display, including MACS and FACS.
• Evaluated nanobody-antigen interactions and performed downstream validation of candidate binders.
• Worked with recombinant antibodies and antibody fragments, including scFv and Fab proteins.
• Maintained accurate experimental records and analyzed experimental results to guide subsequent experiments.
• Applied sterile and aseptic laboratory techniques and followed established laboratory safety and research protocols.
• Independently troubleshot experimental procedures and optimized protocols based on protein behavior and experimental results.
• Collaborated with researchers and mentors to plan experiments, interpret data, and advance ongoing research projects.

Bachelor of Science - Biochemistry - Washington State University (2023)