P L

Experienced Analytical Scientist with 10+ years in biologics and gene therapy, specializing in method development, qualification, and technology transfer. Proven expertise in Mass Photometry, AUC, SEC-MALS, DLS, CE-SDS, IEX, SDS-PAGE, and PCR. Demonstrated success in QC testing, GLP-compliant assay development, and presenting complex data to cross-functional teams and external partners.

Skilled in troubleshooting, assay optimization, and supporting regulatory submissions (IND, stability, release).

Analytical Development & QC Scientist – AAV Physical and Chemical Characterization at Franklin Biolabs (Transitioned from UPenn Gene Therapy Program) (2024-10 – Present)

• Lead execution of QC assays including compendial assays, DLS, Mass Photometry, AUC, CE-SDS, SEC-MALS, and SDS-PAGE for AAV-based therapeutics
• Leveraged Mass Photometry for root cause analysis of AAV process deviations
• Authored CE-SDS pre-qualification report to characterize VP ratios and impurities in AAV products
• Authored a white paper comparing Mass Photometry and AUC for AAV capsid content characterization, highlighting relative advantages in throughput, resolution, and regulatory alignment

Analytical Development & QC Scientist – AAV Physical and Chemical Characterization at University of Pennsylvania Gene Therapy Program (2022-06 – 2024-09)

• Developed and pre-qualified CE-SDS and Mass Photometry methods for AAV capsid identity, impurity profiling, and capsid content quantification (Empty/Partial/Full/Overfull) in alignment with ICH guidelines
• Oversaw tech transfer and GLP qualification of Mass Photometry assay at client CDMO
• Characterized AAV, DNA, and LNP samples using DLS, AUC, SEC-MALS, LC-MS, and CE-SDS
• Designed and transferred AEX assay to MS&T to serve as a predictive model for large-scale purification
• Authored SOPs for Mass Photometry, SEC-MALS, CE-SDS, and SDS-PAGE assays
• Delivered technical presentations to stakeholders including project teams, QA, and external collaborators
• Performed QC testing for release, toxicology, and stability studies under GLP
• Consulted on data and sample tracking management systems using Veeva and LIMS

Analytical Development Associate – AAV Chemical and Biochemical Characterization at Spark Therapeutics, Inc. (2021-11 – 2022-06)

• Developed and pre-qualified multiple SEC-MALS methods; successfully transferred to QC for routine use
• Performed method optimization to enhance analytical performance and throughput of SEC-MALS assays
• Conducted routine and non-routine testing on experimental gene therapy drug substances

Research And Development Scientist – Immuno-Diagnostics at Thermo Fisher Scientific (2021-07 – 2021-11)

• Supported product development for LabType diagnostic assays
• Investigated and resolved customer-reported assay discrepancies
• Optimized PCR, primer design, conjugation, hybridization, and data analysis to improve assay robustness

Research Assistant – Fungal Secondary Metabolites/Natural Products at University of Southern California (2018-01 – 2020-08)

• Engineered fungal biosynthetic pathways to investigate enzyme-substrate relationships
• Purified and analyzed novel fungal metabolites using LC-MS, NMR, and semi-synthesis
• Analyzed NGS data to identify unexplored gene clusters associated with secondary metabolite production

Research Assistant – Peptide Chemistry at University of Southern California (2017-08 – 2018-01)

• Synthesized over 20 peptides and cyclotides via solid-phase peptide synthesis for structure-activity studies
• Applied SEC, IEX, and HPLC-MS for purification and characterization
• Explored receptor-ligand interactions using FRET and E. coli-based protein expression systems

Research Assistant – Computational Biochemistry at Fox Chase Cancer Center (2016-06 – 2017-05)

• Developed Python-based tools for large-scale structural analysis of kinase crystal structures
• Used machine learning to classify kinase inhibitor interactions based on structural features
• Conducted docking simulations to guide development of novel kinase inhibitors

Laboratory Technician – Organic Synthesis at Thomas Jefferson University (2016-08 – 2017-05)

Performed various chemical synthesis to support collaborative biological studies

M.S. in Pharmaceutical Science – University of Southern California (2020-08)

B.S. in Pre-Medical Studies (minor in chemistry) – Philadelphia University (2017-05)