Siva Sangari

Ph.D.-trained immunology and molecular biology scientist transitioning into regulatory and clinical regulatory functions, with a strong foundation in translational research, preclinical studies, biomarker development, assay development, single-cell genomics, multi-omics, and scientific documentation. Experienced in interpreting complex experimental datasets, reviewing scientific evidence, preparing manuscripts and technical materials, collaborating across research teams, and communicating findings clearly to scientific audiences. Formal training in Good Clinical Practice (GCP), clinical trial and statistical methodology, drug discovery and development, and AI applications in healthcare.

Interested in Regulatory Scientist, Regulatory Affairs, Clinical Regulatory, Regulatory/Scientific Writing, and GCP/Clinical Compliance positions.

Independent Researcher / Scientific Consultant - Independent Professional Development - Boston, MA

(2025-03)

• Provide scientific consulting support for preclinical study workflow design, in vivo model planning, multi-omics interpretation, and experimental troubleshooting.
• Review and edit manuscripts, grants, and technical reports for scientific clarity, consistency, rigor, and effective communication of evidence.
• Develop working knowledge relevant to regulated research environments through training in GCP, clinical trial and statistical methodology, drug discovery and development, and AI applications in healthcare.
• Translate complex experimental and multi-omics findings into clear scientific narratives for research and translational applications.

Postdoctoral Research Associate - Tufts University School of Medicine, Department of Immunology - Boston, MA

(2023-02 - 2024-09)

• Applied miR-eCLIPse technology to characterize miRNA–mRNA interactions in renal injury and generated mechanistic evidence supporting NIH grant renewal.
• Defined molecular pathways associated with antibody-mediated renal injury using Ingenuity Pathway Analysis and integrated molecular and cellular datasets.
• Optimized three validated methods for primary glomeruli single-cell isolation and generated datasets using confocal microscopy, flow cytometry, and RNA-seq workflows.
• Performed primary immune-cell and mammalian cell culture, multicolor flow cytometry, RT-qPCR, Western blotting, ELISA/MSD-based assays, and molecular profiling.
• Documented experimental workflows, analyzed results, prepared scientific materials, and communicated findings with collaborators and trainees.
• Mentored eight master’s students and supported collaborative research projects involving experimental design, data analysis, and scientific communication.

Postdoctoral Research Fellow - University of Illinois Chicago, Department of Microbiology & Immunology - Chicago, IL

(2021-02 - 2022-04)

• Investigated OX40L–Jagged1 signaling and Treg biology in autoimmune disease models, including lupus nephritis.
• Developed and evaluated cellular and molecular assays using primary immune cells, flow cytometry, ELISA, qPCR, Western blotting, and immunofluorescence.
• Analyzed and interpreted experimental datasets using GraphPad Prism and pathway-based approaches and communicated findings through manuscripts, presentations, and collaborative research.
• Supported experimental planning, in vivo studies, tissue collection, and interpretation of immune phenotypes.

Doctoral & Postdoctoral Researcher - Bharathidasan University - India

(2010-04 - 2016-10)

• Conducted biochemical and molecular research involving inflammation, metabolic disease, cellular signaling, and tissue injury.
• Performed mammalian cell culture, RNA/DNA isolation, RT-qPCR, Western blotting, ELISA, immunocytochemistry, protein assays, and data analysis.
• Developed experimental workflows, maintained research records, interpreted complex datasets, prepared manuscripts, and presented scientific findings.

Junior & Senior Research Fellow - DBT-Sponsored Research Project - India

(2011 - 2014)

• Supported federally funded research activities involving experimental design, molecular biology assays, data collection, analysis, and scientific reporting.
• Maintained research documentation and contributed to presentations, publications, and project deliverables.

Ph.D. - Biochemistry - Bharathidasan University, India (2016)

M.Phil. - Biochemistry - SASTRA University, India (2009)

M.Sc. - Biochemistry - SASTRA University, India (2008)

B.Sc. - Biochemistry - Mother Teresa Women’s University, Kodaikanal, India (2006)