Metagenomi
Research Associate II Feb 2022 - Present
- Novel knock out candidate identification for cell therapy product (scRNAseq, bulkRNAseq, etc)
- Advanced CAR-T and Gene Therapy cell engineering using novel genome editing toolbox and multi-delivery methods (LNP, EP)
- Testing and screening new genome editing systems (Type II, Type V, and Type VI enzymes); designing sgRNA
- Off-target analysis (both in-lab and in silico) for specificity and efficiency of gene editing
- Cell based functional assays for characterization of therapeutic efficacy (ELISA, luciferase, western, ddPCR)
- Designed sophisticated scRNAseq analysis pipeline (python and R based tools implemented)
Genentech
Scientific Researcher Feb 2021 - Feb 2022
- Working to develop spatial transcriptomics pipeline
- High Order multiplexing (TSA amp, HQ amp, etc)
- Imaging Mass Cytometry experience (Hyperion/Helios)
- RNAseq library preparation (qPCR, Bioanalyzer, Qubit, Tapestation)
- Novel method and assay development
Unity Biotechnology
Research Associate and Research Associate II September 2019-Feb 2021
- IHC Chromogenic and IF multiplexing (6 colors) in human and rodent tissue
- Data from in vivo experiments went into two successful IND filings
- Antibody validation and assay development for target identification
- RNA isolation and qPCR for gene expression detection
- ELISA, Western blot, and other functional cell based assays
UCSC Don Smith METX Lab
Undergraduate Researcher December 2018-Jan 2020
- Routinely performed intraperitoneal injections, dissected and sectioned neuronal tissue, harvested blood
- Worked on year long neurobehavioral study with Long Evans rats (Publication in progress)
- Skills Utilized: qPCR, nanodrop, flow cytometry, western’s, IHC, necropsy, HPLC-MS, bulk RNAseq
