Xiaohua Tian

M.D., Ph.D. (U.S. citizen)

Talent

234 Highland Lake Circle, Decatur, GA 30033Member since May 18, 2026

RNA/DNA/protein Extraction from cells, tissues, virus and bacteria, and quantifiPCRReal-time PCRDNA and RNA library preparation for NGSWestern blottingImmunoprecipitationTissue sectioning and embedding (frozen and paraffin)H&E stainingELISAIsolation of primary epithelial cells and endothelial cellsCell cultureCell transfection by electroporationLuciferase analysisAntibody production and purification (in vivo and in vitro)Antibody development using hybridomaQuality control procedures on equipment, reagents and productsDevelop and determine procedures for clinical testsExtraction of DNA from peripheral bloodFlow cytometry crossmatch and PRAImmunophenotyping using BD FACS CaliburDNA typing of HLA Class I and class II by PCR-SSPDNA typing by reverse sequence specific oligonucleotides (rSSO)Luminex (LabScan 100)Antibody screening (PRA) by LuminexCernerMicrosoft WordExcelPhotoshopPower pointInvitrogen Tray

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RNA/DNA/protein Extraction from cells, tissues, virus and bacteria, and quantifiPCRReal-time PCRDNA and RNA library preparation for NGSWestern blotting

About

Performing molecular diagnoses using PCR and RT-PCR and NGS.

Experience

Senior technologist - Labcorp MNG lab - Atlanta, GA

(2024-01)

Performing molecular diagnoses using PCR and RT-PCR and NGS.

Senior scientist and Production Manager - Caprico Biotech Corp - Atlanta, GA

(2020-07 - 2024-12)

Antibody production from in vivo or in vitro

HLA technologist - MD Anderson Cancer Center HLA Lab - Houston, TX

(2019-06 - 2020-06)

Performing HLA typing for transplant patient.

Senior Scientist - Omega Biotek Company - Atlanta, GA

(2016-05 - 2019-05)

Performing Next generation sequencing with Miseq including DNA and RNA library prep and sequencing

HLA Technologist - Georgia Regents University Health Center - Augusta, GA

(2014-06 - 2016-05)

Performing HLA typing for transplant patient.

Histocompatibility Technologist - University of Chicago Hospital Histocompatibility and Immunogenetics Lab

(2012-07 - 2014-06)

Research Associate - Morehouse School of Medicine, Cardiovascular Research Institute

(2009-10 - 2012-06)

Research on NCF-1 and its pseudogene alternative splicing isoforms; and methylation of RNA and DNA related to cardiovascular diseases.

Performing experiments, supervising Ph.D students, and drafting manuscripts for research paper and proposals.

Postdoctoral Research - Tulane University Health Science Center Department of Medicine Section of Pulmonary Diseases - New Orleans, LA

(2006-11 - 2009-09)

Research on "miRNA modulates epithelial-mesenchymal transition in lung fibrosis and cancer", and "Regulative mechanisms of G protein-coupled receptors in chlorine-induced acute lung injury" using molecular biological techniques and animal experiments.

Postdoctoral Researcher/Instructor - Louisiana State University Medical Center Neuroscience Center

(1998-05 - 2006-10)

Researching on "Molecular biological mechanisms of bioactive lipids in brain ischemia", and "Preclinical study on compounds (PAF-receptor antagonists) with mouse model of cerebral ischemia /reperfusion".