Midalo Ntsieni

Microbiology graduate with hands-on experience in fermentation, microbial quality control, contamination investigations, and applied microbiology research. Experienced in culturing and identifying microorganisms, maintaining microbial strains, analysing growth data, and supporting ISO/IEC 17025-compliant laboratory activities. Contributed to fermentation projects, root-cause investigations, and microbiological testing using both classical and molecular techniques.

Strong foundation in laboratory documentation, aseptic practices, and quality-focused microbiological work.

Candidate Intern - UVU Bio

(2025-08 - 2025-12)

• Executed a 20 L lager beer fermentation from wort preparation through bottling, achieving 4.99% ABV within specification while maintaining GMP-aligned sanitation standards.
• Investigated microbial contamination using selective culturing, 16S rRNA PCR, and restriction digestion, tracing Enterobacteriaceae contamination to a biofilm source and supporting corrective actions.
• Monitored E. coli growth kinetics through OD600 analysis, documenting a 15-fold increase in cell density and interpreting fermentation data to evaluate microbial performance.
• Maintained microbial cultures, prepared inocula, and applied aseptic techniques to support fermentation and quality-control activities.
• Documented laboratory activities analysed experimental results, and generated reports in accordance with ISO/IEC 17025 requirements.

Student Laboratory Assistant - University of Limpopo

(2023-04 - 2023-10)

• Supported continuous improvement initiatives through root-cause investigations, laboratory housekeeping, equipment maintenance, and adherence to laboratory safety procedures
• Supported microbiology practical training across multiple laboratory sessions, ensuring accurate experimental setup and compliance with laboratory safety procedures.
• Guided students in aseptic techniques, sterile transfers, and core microbiological methods, reinforcing good laboratory practices.
• Assisted with laboratory readiness by preparing materials, monitoring equipment availability, and maintaining organized workspaces.
• Reviewed laboratory reports for accuracy and scientific reasoning while providing constructive feedback to support student development.

BSc Honours - Microbiology - University of Limpopo (2023)

BSc - Life Sciences (Microbiology & Biochemistry) - University of Limpopo (2022)