Tshepiso Mpitsi
Laboratory Assistant
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About
Biotechnologist with a Diploma in Biotechnology from the University of Johannesburg and 1.5 years of laboratory experience. Experienced in molecular biology techniques including DNA extraction, PCR, gel electrophoresis, enzymatic purification, and Sanger sequencing preparation. Strong understanding of Good Laboratory Practices (GLP), Standard Operating Procedures (SOPs), quality control, and laboratory safety compliance.
Detail-oriented, adaptable, and committed to operational efficiency and continuous improvement.
Experience
Laboratory Assistant at Inqaba Biotechnical Industries (2023-07 – 2024-12)
Performed genomic DNA extraction, PCR amplification, gel electrophoresis, and sample preparation for Sanger sequencing while maintaining strict adherence to GLP and SOP requirements.
- Performed genomic DNA extraction from diverse biological samples
- Conducted PCR amplification and direct PCR for genetic analysis
- Performed gel electrophoresis for PCR product quality control
- Executed enzymatic and gel purification processes
- Prepared samples for Sanger sequencing
- Maintained accurate laboratory documentation and inventory records
- Ensured strict adherence to GLP and SOP requirements
- Managed micropipette tip preparation, autoclaving, and laboratory equipment maintenance
- Controlled microwaste disposal in compliance with environmental and safety regulations
Education
Diploma in Biotechnology – University of Johannesburg (2024)
National Senior Certificate – Phehello High School (2019)
