Effi Haque

I hold a Ph.D. in Molecular Biology and have extensive experience in creating recombinant mammalian cell lines, particularly in the context of cancer research and drug development. My current work as postdoc at Trinity College Dublin focused on cell biology, genetics, and epigenetics, with a particular emphasis on clear cell sarcomas in children's. I have established proficiency in mammalian cell culture techniques and molecular biology methods, including vector design, construction, and mammalian cell transfection.

I am skilled in using various protein assay techniques for screening transfected cells, including immunoprecipitation, western blotting, and activity-based probes. Additionally, I have experience with flow cytometry, protein analysis, and the use of confocal microscopy for imaging. I have also worked on the development of cell models for testing novel therapeutic approaches, integrating cutting-edge technologies such as CRISPR-Cas9 genome engineering and high-throughput RNAseq analysis.

I am familiar with regulatory standards for GMP manufacturing and have worked in multidisciplinary environments with a focus on precision diagnostics and therapeutic development. My expertise, combined with a strong foundation in molecular and cell biology, positions me to make significant contributions to cell-based therapeutic research and cell line development.

Postdoctoral Researcher - Trinity College Dublin - Dublin, Ireland

(2024-01)

Paediatric Solid Tumour Biology Research group, Trinity Translational Medicine Institute (TTMI)

• Developed and optimized experimental protocols based on previously published articles to address the specific research goals.
• Independently performed experiments to validate and adapt new protocols, ensuring high-quality results aligned with our group's objectives.
• Successfully generated reliable data, contributing to the advancement of ongoing research projects.
• Collaborated with different institute/ lab/ team members for experimental purposes, to troubleshoot and refine protocols.
• Contributed to the interpretation of results and the presentation of findings in group meetings.

Postdoctoral Researcher - The International Institute of Molecular Mechanisms and Machines (IMol) - Poland

(2023-03 - 2023-12)

Polish Academy of Sciences

• Responsible for all molecular and cell biology related experiments.
• Validation of newly synthesized chemical probes that enable high-throughput analysis and exploration of the complex biological networks involved in Probe-Protein interactions in live cells, Continue to lead a project where I approach to investigate biologically significant polyamine interactions with proteins that have not been addressed before.
• Lab members training in molecular and cell biology techniques.
• Presentation of research data during weekly and monthly meetings.

Ph.D. fellow - Institute of Genetics and Animal Biotechnology of Polish Academy of Sciences - Poland

(2018-10 - 2022-10)

Project name: NCN OPUS 13 (2017/25/B/NZ5/02762). Investigating the effects of transcription factor mutations in liver cancer development.

• Cloned gene of interest into a plasmid vector and then mutagenesis.
• Identified the transcriptional activity of the mutated genes.
• Observed nuclear localization by Immunofluorescent Staining.
• Analyzed the protein-protein interaction by Co-immunoprecipitation and the DNA binding ability using EMSA.
• Generate KO/KI cell line using the CRISPR-Cas9 technique.
• Generate the KO embryo of a mouse using the CRISPR-Cas9 technique.
• Overexpression of genes by electroporation and differential gene regulation analysis by qPCR and RNA-sequence.
• Organization and presentation of experimental data for the funding submission and conferences.
• Experienced in developing and optimizing experimental protocols based on previously published articles to address specific research goals.
• Successfully generated reliable data, contributing to the advancement of ongoing research projects.
• Experienced in collaborating with different institute/ lab/ team members for experimental purposes, to troubleshoot and refine protocols.
• Experienced in molecular and cell biology-related experiments.
• Experienced in validating newly synthesized chemical probes that enable high-throughput analysis and exploration of the complex biological networks involved in Probe-Protein interactions in live cells.
• Experienced in training the new lab members in molecular and cell biology techniques.
• Experienced in presenting research data during weekly and monthly meetings and conferences.
• Experienced in gene cloning into a plasmid vector and then mutagenesis.
• Experienced in confocal microscopy and immunofluorescence techniques.
• Experienced in analyzing the protein-protein interaction by Co-immunoprecipitation and the DNA binding ability using EMSA.
• Experienced in Generating KO/KI cell lines and mouse embryos using the CRISPR-Cas9 technique.

Research Associate - Bangabandhu Sheikh Mujibur Rahman Agricultural University - Gazipur-1706, Bangladesh

(2014-07 - 2014-12)

Project name: Enrichment of Facilities to Promote Quality Higher Education and Research in Biotechnology (World Bank and the Higher Education Quality Enhancement Project (HEQEP) CP # 2071.

• Isolation and molecular characterization of plant probiotic bacteria
• Grant proposal writing.
• Molecular characterization of bacteria-isolated plants, soil, water as well as other environments.
• Performed plant growth and development-related bioassay.
• Analyzed statistical data, wrote reports, and published research findings.
• Communicated with research scientists, organized seminars/conferences, and invited researchers.

Research Associate - Bangabandhu Sheikh Mujibur Rahman Agricultural University - Gazipur-1706, Bangladesh

(2013-07 - 2014-06)

Project name: Discovery of Plant Probiotics and Their Usage as Biofertilizers and Biopesticides for Environment-Friendly Low Input Sustainable Agriculture (Ministry of Science and Technology, Government of the People's Republic of Bangladesh).

• Gathered with project-related personnel.
• Governed laboratory consumables to ensure that stock is ordered on time.
• Collected raw data and analyzed statistical data during publication.
• Achieve training in using common molecular techniques (PCR, electrophoresis, DNA sequencing, and bioinformatics analyses) required for microbiome analysis and elucidation of the underlying molecular mechanism of plant-microbe interactions under both in vitro and in vivo greenhouse conditions.
• Expert in bioassay-guided isolation of bioactive natural products from plants and microorganisms.
• Expert in isolation, culture, and identification of microorganisms by molecular techniques.
• Achieve training in developing bioassay methods for testing the activities of natural and environmental chemical compounds toward various cells.
• Achieve training in Thin-layer chromatography (TLC) which is very useful for separating non-volatile mixtures.

Graduate Teaching Assistant - East-West University - Dhaka, Bangladesh

(2015-01 - 2017-08)

• Assisted faculty members with instructional and lecture preparation of molecular biology courses, delivery, and curriculum development and assessment.
• Assisted in teaching undergraduate students several molecular and cellular biology courses.
• Facilitated classroom, laboratory, and discussion sessions and monitored students in the laboratory to help them with the research work.
• Answered student papers and exams and discussed with students to solve their problems.
• Pioneer of developing the laboratory protocol of the new department, optimization of protocol, and taught the students about the basic laboratory technique.
• Expert in developing trust and rapport with students, engaging the students in learning activities, and developing the ability to maintain positive behaviors with students to optimize their understanding of lessons.
• Ensured student safety standards when I conducted laboratory classes in molecular cell biology.
• Supported students in reaching intellectual and development milestones, ranging from lab training and social skills progress to standardized test score improvements of students.

Ph.D. - Molecular Biology - Institute of Genetics and Animal Biotechnology of Polish Academy of Sciences

Master's - Bangabandhu Sheikh Mujibur Rahman Agricultural University (2012-05 - 2013-12)