Postdoctoral Researcher - Trinity College Dublin - Dublin, Ireland
(2024-01)
Paediatric Solid Tumour Biology Research group, Trinity Translational Medicine Institute (TTMI)
- Developed and optimized experimental protocols based on previously published articles to address the specific research goals.
- Independently performed experiments to validate and adapt new protocols, ensuring high-quality results aligned with our group's objectives.
- Successfully generated reliable data, contributing to the advancement of ongoing research projects.
- Collaborated with different institute/ lab/ team members for experimental purposes, to troubleshoot and refine protocols.
- Contributed to the interpretation of results and the presentation of findings in group meetings.
Postdoctoral Researcher - The International Institute of Molecular Mechanisms and Machines (IMol) - Poland
(2023-03 - 2023-12)
Polish Academy of Sciences
- Responsible for all molecular and cell biology related experiments.
- Validation of newly synthesized chemical probes that enable high-throughput analysis and exploration of the complex biological networks involved in Probe-Protein interactions in live cells, Continue to lead a project where I approach to investigate biologically significant polyamine interactions with proteins that have not been addressed before.
- Lab members training in molecular and cell biology techniques.
- Presentation of research data during weekly and monthly meetings.
Ph.D. fellow - Institute of Genetics and Animal Biotechnology of Polish Academy of Sciences - Poland
(2018-10 - 2022-10)
Project name: NCN OPUS 13 (2017/25/B/NZ5/02762). Investigating the effects of transcription factor mutations in liver cancer development.
- Cloned gene of interest into a plasmid vector and then mutagenesis.
- Identified the transcriptional activity of the mutated genes.
- Observed nuclear localization by Immunofluorescent Staining.
- Analyzed the protein-protein interaction by Co-immunoprecipitation and the DNA binding ability using EMSA.
- Generate KO/KI cell line using the CRISPR-Cas9 technique.
- Generate the KO embryo of a mouse using the CRISPR-Cas9 technique.
- Overexpression of genes by electroporation and differential gene regulation analysis by qPCR and RNA-sequence.
- Organization and presentation of experimental data for the funding submission and conferences.
- Experienced in developing and optimizing experimental protocols based on previously published articles to address specific research goals.
- Successfully generated reliable data, contributing to the advancement of ongoing research projects.
- Experienced in collaborating with different institute/ lab/ team members for experimental purposes, to troubleshoot and refine protocols.
- Experienced in molecular and cell biology-related experiments.
- Experienced in validating newly synthesized chemical probes that enable high-throughput analysis and exploration of the complex biological networks involved in Probe-Protein interactions in live cells.
- Experienced in training the new lab members in molecular and cell biology techniques.
- Experienced in presenting research data during weekly and monthly meetings and conferences.
- Experienced in gene cloning into a plasmid vector and then mutagenesis.
- Experienced in confocal microscopy and immunofluorescence techniques.
- Experienced in analyzing the protein-protein interaction by Co-immunoprecipitation and the DNA binding ability using EMSA.
- Experienced in Generating KO/KI cell lines and mouse embryos using the CRISPR-Cas9 technique.
Research Associate - Bangabandhu Sheikh Mujibur Rahman Agricultural University - Gazipur-1706, Bangladesh
(2014-07 - 2014-12)
Project name: Enrichment of Facilities to Promote Quality Higher Education and Research in Biotechnology (World Bank and the Higher Education Quality Enhancement Project (HEQEP) CP # 2071.
- Isolation and molecular characterization of plant probiotic bacteria
- Grant proposal writing.
- Molecular characterization of bacteria-isolated plants, soil, water as well as other environments.
- Performed plant growth and development-related bioassay.
- Analyzed statistical data, wrote reports, and published research findings.
- Communicated with research scientists, organized seminars/conferences, and invited researchers.
Research Associate - Bangabandhu Sheikh Mujibur Rahman Agricultural University - Gazipur-1706, Bangladesh
(2013-07 - 2014-06)
Project name: Discovery of Plant Probiotics and Their Usage as Biofertilizers and Biopesticides for Environment-Friendly Low Input Sustainable Agriculture (Ministry of Science and Technology, Government of the People's Republic of Bangladesh).
- Gathered with project-related personnel.
- Governed laboratory consumables to ensure that stock is ordered on time.
- Collected raw data and analyzed statistical data during publication.
- Achieve training in using common molecular techniques (PCR, electrophoresis, DNA sequencing, and bioinformatics analyses) required for microbiome analysis and elucidation of the underlying molecular mechanism of plant-microbe interactions under both in vitro and in vivo greenhouse conditions.
- Expert in bioassay-guided isolation of bioactive natural products from plants and microorganisms.
- Expert in isolation, culture, and identification of microorganisms by molecular techniques.
- Achieve training in developing bioassay methods for testing the activities of natural and environmental chemical compounds toward various cells.
- Achieve training in Thin-layer chromatography (TLC) which is very useful for separating non-volatile mixtures.
Graduate Teaching Assistant - East-West University - Dhaka, Bangladesh
(2015-01 - 2017-08)
- Assisted faculty members with instructional and lecture preparation of molecular biology courses, delivery, and curriculum development and assessment.
- Assisted in teaching undergraduate students several molecular and cellular biology courses.
- Facilitated classroom, laboratory, and discussion sessions and monitored students in the laboratory to help them with the research work.
- Answered student papers and exams and discussed with students to solve their problems.
- Pioneer of developing the laboratory protocol of the new department, optimization of protocol, and taught the students about the basic laboratory technique.
- Expert in developing trust and rapport with students, engaging the students in learning activities, and developing the ability to maintain positive behaviors with students to optimize their understanding of lessons.
- Ensured student safety standards when I conducted laboratory classes in molecular cell biology.
- Supported students in reaching intellectual and development milestones, ranging from lab training and social skills progress to standardized test score improvements of students.
