Highly Skilled (Research – Bioinformatics & Plant Genomics) - ICAR – Directorate of Onion and Garlic Research (DOGR) - Rajgurunagar, Pune, MH
(2025-10)
Conducted genome-wide analysis of WRKY transcription factors in onion under chromium and salinity stress using integrated wet-lab (RT-qPCR) and bioinformatics approaches.
- Conducted genome-wide analysis of WRKY transcription factors in onion under chromium and salinity stress using integrated wet-lab (RT-qPCR) and bioinformatics approaches (TBtools, MEGA, MEME, STRING)
- Performed genome-wide identification and characterisation of the WRKY gene family in Allium cepa under abiotic stress conditions
- Carried out in-silico annotation, including chromosomal localisation, gene duplication, synteny, collinearity, and Ka/Ks analysis
- Constructed phylogenetic trees and analysed conserved motifs, gene structure, and physicochemical properties
- Identified cis-regulatory elements (PlantCARE), predicted subcellular localisation, and performed GO enrichment analysis
- Developed protein–protein interaction (PPI) networks using STRING and Cytoscape
- Performed RNA isolation, cDNA synthesis, and RT-qPCR-based expression profiling
- Applied statistical analysis and visualised results using TBtools
- Integrated wet-lab and computational approaches to identify candidate genes for abiotic stress tolerance
- Performed 16S rRNA gene-based microbial identification and diversity analysis
- Isolated microbial DNA and conducted PCR amplification of the 16S rRNA gene using universal primers
- Analysed sequencing data for taxonomic classification and phylogenetic relationships
- Constructed phylogenetic trees using MEGA to study evolutionary relationships among microbial species
- Conducted sequence alignment and similarity analysis (BLAST, NCBI databases)
- Assisted in studying microbial communities associated with plant stress conditions
Research Scholar (MSc) - ICAR – Directorate of Onion and Garlic Research (DOGR) - Pune, MH
(2024-02 - 2024-02)
- Performed genome editing experiments, including molecular cloning, plant transformation, and regeneration of transgenic plants
- Designed and optimised CRISPR–Cas guide RNAs for targeted genome editing
- Designed gene-specific primers and optimised PCR and RT-PCR conditions
- Analysed Sanger sequencing data for mutation detection and sequence validation
- Processed and interpreted Next-Generation Sequencing (NGS) datasets
- Conducted phylogenetic analysis and gene structure prediction using bioinformatics tools
- Retrieved, curated, and analysed biological data from public databases (NCBI, UniProt, etc.)
Internship Researcher - CSIR – National Chemical Laboratory (NCL) - Pune, MH
(2023-06 - 2023-06)
- Worked on computer-aided drug discovery (CADD) projects
- Performed virtual screening and molecular docking to evaluate ligand–protein interactions
- Analysed computational simulation data for lead compound optimisation
- Collaborated with interdisciplinary teams, integrating computational and biochemical insights
Training Researcher (B.Tech) - ICAR – National Institute of Abiotic Stress Management (NIASM) - Baramati, MH
(2022-01 - 2022-01)
- Hands-on training in the Animal Biotechnology laboratory and animal experimentation facilities
- Exposure to animal housing systems, data recording, and ethical handling
- Worked in the hydroponics unit growing maize, oats, barley, wheat, rice, and sorghum
- Conducted fermentation and silage production for animal feed
- Estimated biochemical parameters, including BUN, AST, ALT, calcium, and phosphorus
- Participated in conferences on Animal Nutrition, Physiology & Breeding
