Saimanoj Kumar
Production Executive
Talent
HyderabadMember since September 21, 2026
End-point PCRColony PCRReal-Time PCR (qPCR)SYBRTaqManGradient PCRRT-PCRcDNA synthesisSDS-PAGEGel ElectrophoresisLowry MethodAgarose GelGel ExtractionPCR Clean-upPrimer DesignPrimer3NCBI Primer-BLASTNanodrop spectrophotometerProtein quantificationOD analysisInductionExpressionCell lysisCentrifugationChromatographyBMR documentationGMP complianceMolecular Biology enzyme developmentBuffer formulationProduct formulation
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End-point PCRColony PCRReal-Time PCR (qPCR)SYBRTaqMan
About
Key Focus: Bioprocess + In-Process PCR Techniques.
Experience
Production Executive - Gene to Protein Pvt Ltd
(2025-02)
Key Focus: Bioprocess + In-Process PCR Techniques.
- End-point PCR and Colony PCR & for recombinant clone confirmation.
- Real-Time PCR (qPCR – SYBR & TaqMan): Host cell DNA contamination check.
- Gradient PCR for Tm optimization to ensures specificity.
- RT-PCR & cDNA synthesis.
- QC: SDS-PAGE, Gel Electrophoresis, Lowry Method, Agarose Gel + Gel Extraction & PCR Clean-up.
- Primer Design (Primer3, NCBI Primer-BLAST) for QC primers & troubleshooting primer-dimer.
- Nanodrop spectrophotometer: DNA/RNA and protein quantification.
- Upstream process: Inoculum prep 5L & 100L, OD analysis, induction & expression.
- Downstream process: Cell lysis, Centrifugation, Chromatography, TFF (10/30 kDa) – 5X concentration & diafiltration.
- BMR documentation & GMP compliance.
Research Associate - CoreGenelabs Pvt. Ltd
(2022-02 - 2024-03)
- Molecular Biology enzyme development and optimization
- Buffer formulation, preparation, and optimization
- Development and optimization of Molecular Biology kits
- Product formulation, process development, and production support
- Laboratory experimentation and performance evaluation
- Quality control and product testing activities
- Preparation and maintenance of technical and experimental documentation
- Troubleshooting and optimization of Molecular Biology products and processes
Technical Assistant (Lab Works) - University of Hyderabad
(2021-07 - 2021-12)
- Lab works: media/buffer prep, autoclaving, sterilization.
- Competent cell prep, transformation, DNA/RNA extraction, plasmid isolation, Nanodrop.
- Gel electrophoresis, SDS-PAGE, gel extraction, PCR clean-up, restriction digestion.
- Maintained records & calibration logs.
Education
M.Sc. - Agricultural Biotechnology - SHUATS, Allahabad (2017 - 2019)
B.Sc. - Agricultural Biotechnology - MAU, Parbhani (2013 - 2017)
Intermediate - Sri Chaitanya Jr College, Hyd (2011 - 2013)
10th - SMAG High School, Nizamabad (2011)
Skills
Production Executive
