Sakshi Dhule

Recent M.Sc. Biotechnology graduate (CGPA: 8.5) with 5 months of hands-on preclinical research in a cGLP-regulated environment — quantifying anti-inflammatory cytokines (IL-6, MMP-13) via ELISA, running MTT viability assays across dose-response curves, and maintaining a mammalian cell line (SW982) through a zero-contamination study. Experienced in SOP-driven workflows, deviation logging, and GDP-compliant documentation.

Targeting an entry-level Research Associate Trainee / QC Analyst role in pharma or biopharma.

Dissertation project — Evaluation of the Anti-Inflammatory Potential of Nutraceutical Drug In An In Vitro Synovial Cell Model - Interactive Research School for Health Affairs (IRSHA), BVDU - Pune

(2025-12 - 2026-04)

• Demonstrated anti-inflammatory efficacy of a nutraceutical drug as measured by quantified reduction in IL-6 & MMP13 via ELISA, by designing a full cytokine profiling workflow on LPS-stimulated SW982 cells under cGLP-compliant documentation standards.
• Established IC₅₀ dose-response relationship as measured by cytotoxicity screening across 6 concentration gradients, by running MTT assays validated across 3 biological replicates per SOP guidelines.
• Maintained SW982 synovial cell line across 5 months as measured by zero contamination incidents, using aseptic passaging in LAF cabinet and CO₂ incubator per standard operating procedures.
• Reduced assay preparation errors to zero across 30+ experimental runs by supervisor review, by maintaining a GDP-compliant deviation log for full traceability.

Summer Research Intern — Evaluated Antiglycation Potential of an Ayurvedic Formulation measured by % inhibition - Interactive Research School for Health Affairs (IRSHA), BVDU - Pune

(2025-05 - 2025-06)

• Evaluated anti-glycation potential of an Ayurvedic formulation as measured by % inhibition of AGEs as per SOP, using fluorescence-based assay and ELISA plate reader — all data logged per cGLP standards.

M.Sc. - Biotechnology - Rajiv Gandhi Institute of IT and Biotechnology, BVDU (2026)

B.Sc. - Botany, Microbiology & Chemistry - Rajarshi Shahu Mahavidyalaya, Latur, SRTMU, Nanded (2024)