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Shivani Kinha

Shivani Kinha

M.Sc. Biotechnology | Microbiology | Research & Development | Quality Control | Life Sciences

Talent
GurgaonMember since October 5, 2026
Bacterial isolationserial dilutionspread plate and streak plate methodspure culture maintenancecolony morphologyGram stainingacid-fast (ZN) stainingsurface sterilizationCatalaseoxidasecitrate utilizationureasestarch hydrolysismethyl redhalotolerance (NaCl tolerance) assayGel electrophoresisAseptic techniqueculture media and buffer (PBS) preparationglassware and media sterilizationsterility checkssample handlingbiosafety protocolslaboratory records and documentationAutoclavehot air ovenlaminar air flowbiosafety cabinetincubatorcompound microscope (oil immersion)centrifuge
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Bacterial isolationserial dilutionspread plate and streak plate methodspure culture maintenancecolony morphology

About

M.Sc. Biotechnology graduate (CGPA 7.6/10) with hands-on experience in microbial isolation, pure culture techniques, Gram staining and biochemical characterization. Isolated and characterized 44 endophytic bacterial isolates in a research dissertation and completed a Microbiology & Biosafety internship.

Proficient in aseptic technique, media preparation, sterilization and laboratory documentation. Seeking an entry-level role as Research Assistant, Trainee, Microbiologist, Laboratory Technician, Quality Control / Quality Assurance Analyst or Biotechnology Executive in the biotechnology, pharmaceutical, food, agriculture, healthcare or life-science sector.

Experience

MSc Dissertation – Isolation of Bacterial Endophytes from Halophytic Plant Suaeda sp. at Department of Biotechnology, Kurukshetra University (2026-01 – 2026-06)

Supervisor: Dr. Sulekha Chahal

  • Isolated 44 morphologically distinct endophytic bacteria from root (16), stem (15) and leaf (13) tissues using plant-part inoculation and maceration methods on nutrient agar.
  • Performed surface sterilization with sterility checks, aseptic culturing under laminar air flow, and purified isolates by repeated streaking; maintained pure cultures on agar slants.
  • Characterized isolates by colony morphology and Gram staining (majority Gram-negative rods) and by catalase, oxidase, citrate, urease, starch hydrolysis and methyl red tests.
  • Assessed salt tolerance on nutrient agar with 0–15% NaCl; isolates grew up to 10% NaCl, identifying halotolerant strains.
  • Recorded, tabulated and analyzed experimental observations.

Microbiology & Biosafety Lab Intern at Amity University, Haryana (2025-06 – 2025-07)

  • Assisted in microbiological experiments and microbial culturing.
  • Prepared culture media and sterilized laboratory equipment.
  • Performed acid-fast (ZN) staining and handled samples following biosafety protocols.
  • Maintained aseptic conditions and accurate laboratory records and documentation.

Education

Master of Science (M.Sc.) in Biotechnology – Kurukshetra University, Kurukshetra, Haryana (2024 – 2026-08)

Bachelor of Science (B.Sc.) in Biotechnology – Gurugram University, Gurugram, Haryana (2024-07)

Skills

M.Sc. BiotechnologyMicrobiologyResearch & DevelopmentQuality ControlLife Sciences

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