Ojaswee Dahal

Scientist I

Talent

Olney, MD 20832Member since June 6, 2026

Mammalian Cell cultureLymphoma cell linesRetina cell linesAML cell line HK293TCell TransfectionNucleofectionFlow cytometerBSC-III LAB SettingDNA extractionQubitNanodropAgilent Real-time PCRDroplet digital PCRBioanalyzerCloningCRISPER WITH CAS12Site directed mutagenesisPCRRT PCRPlasmid DNA isolationBAC library constructionFosmid libraryRestriction enzyme digestionLigationTransformationSequencingNGSProtein purificationProtein A/G resinsSpectrophotometric methods

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Mammalian Cell cultureLymphoma cell linesRetina cell linesAML cell line HK293TCell Transfection

About

Highly skilled and dedicated scientist with about 10 years of experience in a biotech company located at Gaithersburg, MD. As an active team member, I have been providing my services to the company in performing the time-bound research projects pertaining to cloning, sequencing, BAC library construction and recombinant antibody technology. I have been actively involved in the isolation of DNA and RNA, restriction enzyme digestion, ligation, transformation, and screening of positive clones.

My involvement in phage display research involves growing and harvesting of phages, titer determination by plaque assay, bio panning, Elisa and phage DNA isolation for cloning and sequencing. Cell culture responsibilities include maintenance of primary and transformed cell lines, transfection, expression evaluation and protein purification.

Experience

Scientist I - Seracare (LGC)

(2022)

Direct and supervise the day-to-day activities of the R&D department. Implement operational policies and procedures to ensure and accuracy of workflows. Use 6s to ensure meeting project goal deadlines and performance.

CELL CULTURE: Proficient in Mammalian Cell culture, lymphoma cell lines, Retina cell lines AML cell line HK293T. Specializing in Adherent and supervision Tumor cell Lines.
Conducted cell Transfection and nucleofection and analyzed the GFP expression in a fluorescence microscope
Maintained the Flow cytometer, worked in a BSC-III LAB Setting
Expert in nucleic acid analysis, encompassing Genomic DNA, RNA, VIRAL RNA, DNA extraction utilizing Qubit/Nanodrop/Agilent Real-time PCR, droplet digital PCR and Bioanalyzer
Cloning vector with the gene of interest. CRISPER WITH CAS12. Site directed mutagenesis, amplification of DNA based on the designed project
Project 1: Quantitative FFPE Blocks
Project 2: Lentiviral (cloning with vector, make Midi prep or Maxiprep DNA, transfection with HEK293T cells, Transduction with mammalian cells, AML cell line, RPE-1 cell line make gDNA, ddpcr: quntasoft1.7.4)
Project 3: AML
Project 4: Brca1 and Brca2 mutations cloned in targeting vector with addition deletions, Transfected that mutations into the GM cells. Likewise CYP2D6 BACS MUTATIONS cloned, make recombineering site, adding plasmid mutations to that site. Analyze the data by digital pcr
Flow cytometer: preparation of samples, maintenance of equipment (Beckman coulter)
Lab maintenance: Decontamination incubator, laminar hood, water bath calibration
AACR PROJECT lab work presentation

Scientist I - Rx Biosciences LLC

(2014 - 2022)

Genomics: Isolation of nucleic acids including plasmid DNA, RNA, genomic DNA from cells, animal tissues and plants
High throughput DNA isolation from BAC and Fosmid library clones
Large scale plasmid DNA isolation including maxi, midi and mega-preps
Set up primers, PCR and RT PCR
Purification of DNA from gel including the PCR products
Setting of restriction enzyme digestion reactions
Native and denaturing DNA and RNA agarose gels
Cloning of DNA in various sequencing and expression vectors
Proteomics: Preparation of cell lysates after transfection, and or induction of mammalian, E. coli and yeast cells
Protein purification using metal affinity and protein A/G resins
Protein concentration estimation by Spectrophotometric methods
Native and denaturing SDS-PAGE
Western Blot Analysis
Protein concentration and preservation
HPLC (received hands-on training)
Covid-19 testing (under CLIA)
Make DNA, RNA and RT-PCR from covid-19
Phage Display Techniques: Maintenance of antibody libraries
Titration and Bio panning
Phage rescue and validation of positive clones by sequencing and ELISA
Cell Biology: Maintenance of transformed and primary adherent and suspension mammalian cells
Transient Transfection
Preparation of cell lysates for protein purification, western blotting and/or immune-precipitation