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Sara Tewoldemedhin

Research Assistant

Talent
Valparaiso, INMember since August 27, 2026
Western blottingBCA protein assayProtein extractionImmunoprecipitationImmunobiotinylationRNA extractionMammalian cell cultureImmunocytochemistryFluorescence microscopyImageJ quantitative image analysisMicropipettingSolution preparationExperimental treatmentsSample preparationQuantitative data analysisANOVAt-testsData interpretationProtocol adherenceExperimental troubleshootingLaboratory documentationAccurate recordkeepingLaboratory safetyOrganizationCollaborative researchExperimental data collectionProtocol development
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Western blottingBCA protein assayProtein extractionImmunoprecipitationImmunobiotinylation

About

Laboratory-focused science graduate with four years of hands-on undergraduate research experience in cellular, molecular, and microbiology-related research. Skilled in mammalian cell culture, immunocytochemistry, Western blotting, BCA protein assays, protein extraction, immunoprecipitation, immunobiotinylation, fluorescence microscopy, RNA extraction, and quantitative data analysis. Experienced in experimental preparation, troubleshooting, detailed laboratory protocols, and accurate documentation.

Experience

Research Assistant - Bouyer Laboratory, Valparaiso University - Valparaiso, IN

(2023-04 - 2026-08)

  • Conduct cellular, molecular, and biochemical experiments under faculty supervision using mammalian epithelial cell models, including T84 and MDCK cells.
  • Perform Western blotting, protein extraction, BCA protein assays, immunoprecipitation, immunobiotinylation, immunocytochemistry, fluorescence microscopy, and RNA extraction.
  • Prepare reagents, solutions, samples, and experimental treatments while following established laboratory protocols and safety procedures.
  • Use fluorescence microscopy and ImageJ to collect and quantitatively analyze experimental imaging data.
  • Contribute to microbiology-related research involving Candida albicans, including experimental treatments, RNA extraction, data collection, and analysis.
  • Assist with protocol development and troubleshoot experimental procedures when results or experimental conditions require further investigation.
  • Maintain organized laboratory records and document experimental procedures, observations, and results to support reproducibility and accurate interpretation of data.
  • Analyze experimental results using quantitative and statistical approaches, including ANOVA and t-tests.
  • Collaborate with faculty and student researchers and communicate experimental findings through scientific posters and research presentations.

Resident Assistant - Office of Residential Life, Valparaiso University - Valparaiso, IN

(2023-07 - 2026-01)

Responding to time-sensitive concerns and emergencies while balancing multiple priorities, following established procedures, maintaining accurate documentation and confidentiality, and demonstrating reliability, organization, attention to detail, and independent judgment in a fast-paced environment.

Orientation Assistant - Student Involvement Center, Valparaiso University - Valparaiso, IN

(2023-06 - 2024-12)

Supported new students and families during orientation by coordinating activities, providing accurate information, resolving concerns, and collaborating with staff in a fast-paced environment.

Event Staff - Harre Union, Valparaiso University - Valparaiso, IN

(2022-12 - 2023-05)

Supported campus events through setup, guest assistance, safety monitoring, problem-solving, and coordination with team members to ensure organized and efficient operations.

Building Manager - Chapel of the Resurrection, Valparaiso University - Valparaiso, IN

(2022-09 - 2023-05)

Managed daily facility operations by assisting guests, coordinating event setup and cleanup, addressing time-sensitive concerns, and maintaining a safe, organized, and professional environment.

Education

Bachelor of Arts - Chemistry and Minor in General Biology - Valparaiso University (2026-08)

Skills

Research Assistant

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