Research Lead at TRIOZ Biosciences (2023-01 – Present)
Direct interdisciplinary research team in developing innovative oncological nutraceutical formulations from discovery through prototype validation, demonstrating project management capabilities essential for independent postdoctoral research.
- Leadership & Team Management: Direct interdisciplinary research team in developing innovative oncological nutraceutical formulations from discovery through prototype validation, demonstrating project management capabilities essential for independent postdoctoral research.
- Manage research operations including budget allocation, timeline coordination, and resource optimization across multiple concurrent projects.
- Mentor junior researchers in experimental design, data analysis, and scientific communication.
- Translational Biotherapeutic Development: Lead end-to-end biotherapeutic development pipeline including formulation design, stability testing, efficacy validation, and quality control for oncologic nutritional formulations.
- Design and execute translational research projects from concept through prototype, bridging basic molecular research with clinical application.
- Establish preclinical validation protocols ensuring therapeutic efficacy and reproducibility.
- Cross-functional Collaboration & Scientific Rigor: Coordinate between molecular biology, clinical nutrition, regulatory affairs, and manufacturing teams to ensure scientific rigor and product efficacy.
- Integrate diverse scientific expertise to advance therapeutic development, demonstrating ability to synthesize multidisciplinary perspectives crucial for translational cardiac research.
- Collaborate with clinical oncologists to align formulation development with patient needs and therapeutic endpoints.
- Grant Writing & Translational Research: Develop research proposals and funding applications to support therapeutic development initiatives.
- Establish standard operating procedures (SOPs) and quality management systems for research operations.
- Translated nutraceutical formulations for cancer from research to market.
Doctoral Researcher at Cancer Research Institute, College of Medicine, Seoul National University (2018-01 – 2021-12)
Focused on molecular mechanisms of disease, protein interactions, and cellular signaling.
- Protein Interaction Discovery: Mapped protein-protein interaction networks in breast cancer using co-immunoprecipitation and LC-MS/MS, identifying novel binding partners and characterizing functional protein complexes.
- Quantitative Mass Spectrometry: Performed mass spectrometry-based proteomics with computational binding site prediction to elucidate molecular mechanisms of protein interactions
- Protein Purification & Validation: Established GST pull-down and Ni-NTA affinity chromatography protocols for recombinant protein isolation and biochemical interaction validation
- Cell Line Engineering: Generated and maintained stable mammalian cell lines with tagged protein expression; optimized transfection protocols for functional studies
- Flow Cytometry Expertise: Conducted multi-parameter FACS analysis for cell sorting, phenotyping, and quantitative cellular assessments
- Advanced Microscopy: Applied immunofluorescence and confocal imaging to visualize protein localization and cellular distribution patterns
- Rigorous Data Analysis: Performed comprehensive statistical analysis including regression modeling, hypothesis testing, and confidence interval determination for publication-quality results.
Research Intern at XBITS, BITS Pilani (2017-01 – 2018-12)
Focused on molecular genotyping expertise, DNA isolation and quality control, genotype-phenotype correlation, and clinical sample processing.
- Multiplexed PCR: Investigated antibiotic resistance mechanisms in urinary tract infections from female patient cohorts by genotyping resistance genes in clinical isolates. Determined prevalence of TEM-1 β-lactamase gene in bacterial populations using multiplexed PCR protocols.
- Nucleic Acid Isolation & Molecular Genotyping: Isolated bacterial DNA from clinical samples using alkaline lysis method. Performed 16S rRNA gene sequencing for bacterial identification and phylogenetic classification. Optimized PCR protocols for detection of antibiotic resistance genes.
- Genotype-Phenotype Correlation: Quantified phenotypic antibiotic resistance using disc diffusion assays (Kirby-Bauer method). Correlated molecular genotyping data with functional phenotypic resistance patterns. Integrated genetic and phenotypic data to establish resistance profiles in clinical isolates.
Academic Trainee at Laboratory of Pathogenesis, University of Hyderabad (2016-01 – 2017-12)
Focused on molecular genotyping expertise, DNA isolation and quality control, genotype-phenotype correlation, and clinical sample processing.
- Advanced Molecular Cloning: Performed molecular cloning of PE/PPE gene family from Mycobacterium tuberculosis in both mammalian and mycobacterial expression systems as master's dissertation project.
- Gateway Cloning Technology: Designed and constructed recombinant clones using Gateway cloning technique, establishing proficiency in site-specific recombinational cloning methodology.
- Clonal Library Construction: Prepared comprehensive clonal library of PE/PPE genes for functional characterization studies.
- Dual Expression Systems: Successfully cloned and expressed genes in both prokaryotic (M. smegmatis) and eukaryotic (mammalian cell lines) expression platforms.
- Protein-Cell Interaction Studies: Investigated the role of PE/PPE proteins in host-pathogen molecular interactions through recombinant protein expression and functional assays.
