Gauri Bhadke
Research professional with 2+ years of experience in molecular biology, mammalian cell culture, protein analysis, and cell-based assays
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About
Research professional with 2+ years of experience in molecular biology, mammalian cell culture, protein analysis, and cell-based assays. Currently working as Project Associate at CSIR-NCL on a national health cohort research program involving biological sample processing and experimental analysis. Experienced in research protocol implementation, laboratory documentation, SOP adherence, data analysis, and scientific reporting.
Skilled in ELISA, PCR/qPCR, immunofluorescence, microscopy, and Western blotting. Strong team collaborator with experience in research coordination and regulatory compliance.
Experience
Project Associate at CSIR–National Chemical Laboratory (NCL) (2025-07 – Present)
- Supported the Phenome India (PI-CHeCK) health cohort project involving human biological samples.
- Lead and independently manage advanced mammalian cell culture workflows, including routine maintenance, passaging, sub-culturing, cryopreservation, revival, and large-scale cell expansion. (Cell lines- CHO, INS1, HepG2, HEK293T, A498, THP1, )
- Execute cell counting, viability, and proliferation assays to assess cellular health and experimental outcomes.
- Conducted in vitro cell-based assays and ELISA for biomarker evaluation.
- Recorded, analyzed, and documented experimental data; prepared reports and presentations.
Project Assistant at Indian Institute of Science Education and Research (IISER) (2024-10 – 2025-04)
- Worked on p53-mediated regulation of LINE1 retrotransposon-derived R-loops.
- Performed DNA/RNA isolation, PCR/qPCR, Western blotting, and immunofluorescence.
- Assisted in microscopy-based analysis and experimental documentation.
- Documented experimental procedures and maintained research records.
- Supported data analysis and result interpretation.
Master's Thesis at Indian Institute of Science Education and Research (IISER) (2023-05 – 2024-05)
- Investigated RNA–DNA hybrid formation and its role in genomic instability and inflammation using mammalian cell models.
- Performed mammalian cell culture, RNA/DNA isolation, and gene expression analysis using PCR, RT-PCR, and qPCR
- Utilized antibody-based R-loop detection (S9.6) and analyzed experimental data to establish links with cancer-associated cellular stress.
- Conducted immunofluorescence and Western blotting to study R-loop formation and DNA damage response pathways.
Education
BS-MS Dual degree – Indian Institute of Science Education and Research (IISER) (2019 – 2024)
HSC – Jawahar Nvodaya Vidyalaya Wardha (2018 – 2018)
SSC – Jawahar Nvodaya Vidyalaya Wardha (2016 – 2016)
