Md Anarul Hoque

My background includes extensive experience in molecular techniques such as vector design, culture maintenance, vector transformation, and gene cloning (Gibson assembly, Golden Gate assembly). I have successfully employed site-directed mutagenesis, directed evolution, and high-throughput screening in various research projects. My analytical expertise encompasses protein purification techniques, including Ni-NTA, IEC, SEC, and AKTA-FPLC, alongside SDS-PAGE, gel electrophoresis, DSF, Western blot, and HPLC-HIC column analysis.

Additionally, I have hands-on experience with NMR, MALDI-TOF, and cell-based functional assays.

In addition to my technical skills, I have significant experience in lab management, including drafting SOPs, maintaining meticulous documentation, ordering supplies, grant writing, and publishing manuscripts. My collaborative nature and leadership capabilities have allowed me to mentor and supervise junior scientists, students, and fellows effectively. Moreover, I am proficient in utilizing analytical software such as SNAP gene, GraphPad, Unicorn, and the Microsoft Office suite to support experimental design and data analysis.

My contributions to published research in esteemed journals, including Applied Environmental Microbiology, ACS Chemical Biology, Analytical Chemistry, and Methods in Enzymology, reflect my commitment to advancing scientific knowledge and innovation. My ability to design, execute, and manage complex projects has consistently resulted in high-quality outcomes delivered within deadlines.

I am confident that my technical expertise, research experience, and dedication to excellence will make me a valuable asset to your team.

Molecular techniques: Gene cloning, PCR, RT-PCR, ddPCR, Chromosomal integration, Gibson-assembly, Golden gate assembly, E.coli Surface display, DNA guided RNA cleavage, Site and random muta-

genesis

Bench Techniques: Cell culture, Sterile techniques, Plasmid cloning and purification, Transfection, Protein Expression and Purification, Biophotometer, Bioreactor, Gel Electrophoresis, SDS-

PAGE, Westernblot, ELISA, Chromatographic techniques, Differential Scanning Fluorime-

try (DSF), NTA, Biorad/AKTA-FPLC, IEC, SEC, HPLC-HIC, GC-MS, Sacnning Electron Mi-

croscopy, TF Filtration

Protein Kinetics: Protein activity, UV/Vis Spectrophotometer, Kcat/Km value analysis

Software Skills: GarphPad Prism, PyMOL, UCSF Chimera, Unicorn for AKTA, Topspin for NMR, MasterFlex for MALDI-TOF, Biocomand for NewBrunswick fermenter, Autodock 4.0, Vina tool, LATEX

(Overleaf), ImageJ, Microsoft Word, Microsoft PowerPoint, Excel and Outlook

Other

Transferable Skils Leadership: Team Management, Self-motivated, Mentor, Organized, Timemanagement,

Collaborative

Communication English (Full professional proficiency) and Bengali (Native proficiency)

PhD in Biochemistry and molecular biology

Masters in Biotechnology

Bachelors in Biotechnology